Wu Yan, Yang Hong, Liu Hong, Yang Zhihua, Zhu Maoxiang, Pan Xiujie, Huang Taisong, Zou Kexing, Bai Jiafeng, Ma Yanyan, Li Jingzhe
Technical Center, China Tobacco Guangxi Industrial Co., Ltd., Nanning 530001, China.
Zhongguo Zhong Yao Za Zhi. 2011 Nov;36(22):3184-8.
To study the in vitro effect of herb components on scavenging harmful components of cigarette smoke such as radicals, polycyclic aromatic hydrocarbons, nitrosamines in vitro, and its reducing effect on cytotoxicity of cigarette smoke.
spectrophotometry was used to examine the scavenging effect of herb components on DPPH free radicals, superoxide anion radical, and hydroxyl radical, and the results were compared with the anti-oxidation of ascorbic acid. Fluorescence spectroscopy was used to examine the scavenging effect of herb components on polycyclic aromatic hydrocarbons. UV spectrophotometry was used to examine the scavenging effect of herb components on volatile nitrosamines. MTT assay was used to examine cytotoxicity of cigarette smoke.
All the herb components showed a certain scavenging effect on DPPH free radicals, superoxide anion radical, hydroxyl radical, polycyclic aromatic hydrocarbons and volatile nitrosamines, espacially the ginkgo biloba extract (GBE), flavonoids of glycyrrhiza, procyanidine, total saponins in ophiopogonin, total saponins of astragalus and epimediun flavonoids. After these six herb components were added to cigarette, the cytotoxicity of cigarette smoke on BEP2D cells was remarkably reduced, by increasing cell survival fraction (SF, %) and mean lethal dose (DML).
The herb components can scavenge harmful components of cigarette smoke such as radicals, polycyclic aromatic hydrocarbons and nitrosamines, which reduce the damage of cigarette smoke on human being.
研究草本成分对香烟烟雾中自由基、多环芳烃、亚硝胺等有害成分的体外清除作用，及其对香烟烟雾细胞毒性的降低作用。
采用分光光度法检测草本成分对DPPH自由基、超氧阴离子自由基和羟自由基的清除作用，并与抗坏血酸的抗氧化作用进行比较。采用荧光光谱法检测草本成分对多环芳烃的清除作用。采用紫外分光光度法检测草本成分对挥发性亚硝胺的清除作用。采用MTT法检测香烟烟雾的细胞毒性。
所有草本成分对DPPH自由基、超氧阴离子自由基、羟自由基、多环芳烃和挥发性亚硝胺均有一定的清除作用，尤其是银杏叶提取物（GBE）、甘草黄酮、原花青素、麦冬总皂苷、黄芪总皂苷和淫羊藿黄酮。将这六种草本成分添加到香烟中后，香烟烟雾对BEP2D细胞的细胞毒性显著降低，细胞存活分数（SF，%）和平均致死剂量（DML）增加。
草本成分可清除香烟烟雾中的自由基、多环芳烃和亚硝胺等有害成分，减少香烟烟雾对人体的损害。
