无标记 3D 成像技术可用于活体组织床内的微观结构、血管和淋巴管成像。
Label-free 3D imaging of microstructure, blood, and lymphatic vessels within tissue beds in vivo.
机构信息
Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.
出版信息
Opt Lett. 2012 Mar 1;37(5):812-4. doi: 10.1364/OL.37.000812.
Abstract
This Letter reports the use of an ultrahigh resolution optical microangiography (OMAG) system for simultaneous 3D imaging of microstructure and lymphatic and blood vessels without the use of an exogenous contrast agent. An automatic algorithm is developed to segment the lymphatic vessels from the microstructural images based on the fact that the lymph fluid is optically transparent. An OMAG system is developed that utilizes a broadband supercontinuum light source, providing an axial resolution of 2.3 μm and lateral resolution of 5.8 μm, capable of resolving the capillary vasculature and lymphatic vessels innervating microcirculatory tissue beds. Experimental demonstration is performed by showing detailed 3D lymphatic and blood vessel maps, coupled with morphology, within mouse ears in vivo.
摘要
这封信件报告了一种超分辨率光学微血管造影（OMAG）系统的使用，该系统可在不使用外源性对比剂的情况下同时对微观结构和淋巴管及血管进行 3D 成像。开发了一种自动算法，可根据淋巴液在光学上是透明的这一事实，从微观结构图像中分割出淋巴管。开发了一种 OMAG 系统，该系统利用宽带超连续谱光源，提供轴向分辨率为 2.3μm 和横向分辨率为 5.8μm，能够分辨毛细血管血管和支配微循环组织床的淋巴管。通过显示体内小鼠耳朵内的详细 3D 淋巴管和血管图，并结合形态学，进行了实验演示。
相似文献
1
Label-free 3D imaging of microstructure, blood, and lymphatic vessels within tissue beds in vivo.无标记 3D 成像技术可用于活体组织床内的微观结构、血管和淋巴管成像。
Opt Lett. 2012 Mar 1;37(5):812-4. doi: 10.1364/OL.37.000812.
2
Lymphatic response to depilation-induced inflammation in mouse ear assessed with label-free optical lymphangiography.通过无标记光学淋巴管造影术评估小鼠耳部脱毛诱导炎症的淋巴反应。
Lasers Surg Med. 2015 Oct;47(8):669-76. doi: 10.1002/lsm.22387. Epub 2015 Jul 29.
3
Supercontinuum light source enables in vivo optical microangiography of capillary vessels within tissue beds.超连续谱光源可实现组织床内毛细血管的活体光学微血管成像。
Opt Lett. 2011 Aug 15;36(16):3169-71. doi: 10.1364/OL.36.003169.
4
Label-free optical lymphangiography: development of an automatic segmentation method applied to optical coherence tomography to visualize lymphatic vessels using Hessian filters.无标记光学淋巴造影术：基于Hessian 滤波器的自动分割方法的开发，用于使用光学相干断层扫描可视化淋巴管。
J Biomed Opt. 2013 Aug;18(8):86004. doi: 10.1117/1.JBO.18.8.086004.
5
Photoacoustic lymphangiography.光声淋巴造影。
J Surg Oncol. 2020 Jan;121(1):48-50. doi: 10.1002/jso.25575. Epub 2019 Jun 4.
6
Optical microangiography provides correlation between microstructure and microvasculature of optic nerve head in human subjects.光学微血管造影提供了人类受试者视神经乳头的微观结构和微血管之间的相关性。
J Biomed Opt. 2012 Nov;17(11):116018. doi: 10.1117/1.JBO.17.11.116018.
7
Label-free volumetric imaging of conjunctival collecting lymphatics ex vivo by optical coherence tomography lymphangiography.通过光学相干断层扫描淋巴管造影术对离体结膜引流淋巴管进行无标记容积成像。
J Biophotonics. 2018 Aug;11(8):e201800070. doi: 10.1002/jbio.201800070. Epub 2018 Jul 13.
8
Three-Dimensional Imaging of Lymphatic System in Lymphedema Legs Using Interstitial Computed Tomography-lymphography.使用间质计算机断层扫描淋巴造影术对淋巴水肿腿部淋巴系统进行三维成像。
Acta Med Okayama. 2017 Apr;71(2):171-177. doi: 10.18926/AMO/54986.
9
In vivo volumetric imaging of vascular perfusion within human retina and choroids with optical micro-angiography.利用光学微血管造影术对人视网膜和脉络膜内的血管灌注进行体内容积成像。
Opt Express. 2008 Jul 21;16(15):11438-52. doi: 10.1364/oe.16.011438.
10
4D optical coherence tomography-based micro-angiography achieved by 1.6-MHz FDML swept source.基于1.6兆赫兹频域锁模扫频光源实现的4D光学相干断层扫描微血管造影术。
Opt Lett. 2015 Apr 15;40(8):1779-82. doi: 10.1364/OL.40.001779.
引用本文的文献
1
Restoration of cervical lymphatic vessel function in aging rescues cerebrospinal fluid drainage.衰老过程中颈部淋巴管功能的恢复可挽救脑脊液引流。
Nat Aging. 2024 Oct;4(10):1418-1431. doi: 10.1038/s43587-024-00691-3. Epub 2024 Aug 15.
2
Deep learning-based label-free imaging of lymphatics and aqueous veins in the eye using optical coherence tomography.基于深度学习的免标记成像在眼部的淋巴管和房水静脉的光学相干断层扫描。
Sci Rep. 2024 Mar 13;14(1):6126. doi: 10.1038/s41598-024-56273-1.
3
Developing a normative database for retinal perfusion using optical coherence tomography angiography.利用光学相干断层扫描血管造影术建立视网膜灌注的标准数据库。
Biomed Opt Express. 2021 Jun 14;12(7):4032-4045. doi: 10.1364/BOE.423469. eCollection 2021 Jul 1.
4
Simultaneous measurements of lymphatic vessel contraction, flow and valve dynamics in multiple lymphangions using optical coherence tomography.使用光学相干断层扫描技术同时测量多个淋巴管节段中的淋巴管收缩、流量和瓣膜动力学。
J Biophotonics. 2018 Aug;11(8):e201700017. doi: 10.1002/jbio.201700017. Epub 2017 Jul 31.
5
label-free lymphangiography of cutaneous lymphatic vessels in human burn scars using optical coherence tomography.利用光学相干断层扫描对人类烧伤瘢痕中的皮肤淋巴管进行无标记淋巴管造影。
Biomed Opt Express. 2016 Nov 2;7(12):4886-4898. doi: 10.1364/BOE.7.004886. eCollection 2016 Dec 1.
6
In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography.利用多普勒光相干断层扫描术对淋巴液流速和容积流量进行体内无标记测量。
Sci Rep. 2016 Jul 5;6:29035. doi: 10.1038/srep29035.
7
Assessment of edema volume in skin upon injury in a mouse ear model with optical coherence tomography.利用光学相干断层扫描技术评估小鼠耳部损伤模型中皮肤的水肿体积。
Lasers Med Sci. 2016 Sep;31(7):1351-61. doi: 10.1007/s10103-016-1984-x. Epub 2016 Jun 9.
8
Microparticle image velocimetry approach to flow measurements in isolated contracting lymphatic vessels.用于测量孤立收缩淋巴管中血流的微粒图像测速方法。
J Biomed Opt. 2016 Feb;21(2):25002. doi: 10.1117/1.JBO.21.2.025002.
9
Lymphatic response to depilation-induced inflammation in mouse ear assessed with label-free optical lymphangiography.通过无标记光学淋巴管造影术评估小鼠耳部脱毛诱导炎症的淋巴反应。
Lasers Surg Med. 2015 Oct;47(8):669-76. doi: 10.1002/lsm.22387. Epub 2015 Jul 29.
10
Label-free optical imaging of lymphatic vessels within tissue beds .组织床内淋巴管的无标记光学成像。
IEEE J Sel Top Quantum Electron. 2014 Mar-Apr;20(2):6800510. doi: 10.1109/JSTQE.2013.2278073.
本文引用的文献
1
Supercontinuum light source enables in vivo optical microangiography of capillary vessels within tissue beds.超连续谱光源可实现组织床内毛细血管的活体光学微血管成像。
Opt Lett. 2011 Aug 15;36(16):3169-71. doi: 10.1364/OL.36.003169.
2
Highly sensitive imaging of renal microcirculation in vivo using ultrahigh sensitive optical microangiography.使用超高灵敏度光学微血管造影术对体内肾微循环进行高灵敏度成像。
Biomed Opt Express. 2011 Apr 1;2(5):1059-68. doi: 10.1364/BOE.2.001059.
3
Three-dimensional optical imaging of microvascular networks within intact lymph node in vivo.体内完整淋巴结内微血管网络的三维光学成像。
J Biomed Opt. 2010 Sep-Oct;15(5):050501. doi: 10.1117/1.3496301.
4
Preclinical lymphatic imaging.临床前淋巴成像。
Mol Imaging Biol. 2011 Aug;13(4):599-612. doi: 10.1007/s11307-010-0421-y.
5
Sentinel lymph nodes and lymphatic vessels: noninvasive dual-modality in vivo mapping by using indocyanine green in rats--volumetric spectroscopic photoacoustic imaging and planar fluorescence imaging.前哨淋巴结和淋巴管：使用吲哚菁绿在大鼠体内进行无创双模态实时成像——容积光谱光声成像和平面荧光成像。
Radiology. 2010 May;255(2):442-50. doi: 10.1148/radiol.10090281.
6
Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging.使用光学频域成像对体内肿瘤微环境进行三维显微镜检查。
Nat Med. 2009 Oct;15(10):1219-23. doi: 10.1038/nm.1971. Epub 2009 Sep 13.
7
Lymphangiogenesis in development and human disease.发育及人类疾病中的淋巴管生成
Nature. 2005 Dec 15;438(7070):946-53. doi: 10.1038/nature04480.
8
In vivo integrated flow image cytometry and lymph/blood vessels dynamic microscopy.体内集成流式图像细胞术和淋巴/血管动态显微镜检查。
J Biomed Opt. 2005 Sep-Oct;10(5):054018. doi: 10.1117/1.2060567.
9
Two-photon imaging of lymphocyte motility and antigen response in intact lymph node.完整淋巴结中淋巴细胞运动性和抗原反应的双光子成像
Science. 2002 Jun 7;296(5574):1869-73. doi: 10.1126/science.1070051. Epub 2002 May 16.
10
Lymphangiogenesis and tumor metastasis: myth or reality?淋巴管生成与肿瘤转移：虚构还是现实？
Clin Cancer Res. 2001 Mar;7(3):462-8.
Zotero 插件