Chang Juhua, Wang Minghua, Gui Wenjun, Zhao Ying, Yu Liang, Zhu Guonian
Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, China.
Zoolog Sci. 2012 Mar;29(3):181-4. doi: 10.2108/zsj.29.181.
The zebrafish (Danio rerio) has been used as a model for the study of endocrine disrupting chemicals. This study set out to determine the profiles of whole-body thyroxine (T4) and 3,5,3'-triiodothyronine (T3) levels during the development of zebrafish from embryo to adult. Enzyme-linked immunoassay was used to analyze whole-body T4 and T3 contents. The results showed that whole-body T4 and T3 levels remained stable during the pre-hatching period (0-3 d) and increased significantly during early development after hatching. The T3 level peaked at 0.28 ± 0.01 ng g(-1) body weight at 10 days post-fertilization (dpf), and T4 peaked at 0.58 ± 0.09 ng g(-1) body weight at 21 dpf. Both thyroid hormones subsequently declined during later development. This study establishes a baseline for thyroid hormones in zebrafish, which will be vital for the understanding of thyroid hormone functions and in future studies of thyroid toxicants in this species.
斑马鱼（Danio rerio）已被用作研究内分泌干扰化学物质的模型。本研究旨在确定斑马鱼从胚胎发育到成体过程中全身甲状腺素（T4）和3,5,3'-三碘甲状腺原氨酸（T3）水平的变化情况。采用酶联免疫分析法分析全身T4和T3含量。结果表明，在孵化前期（0 - 3天）全身T4和T3水平保持稳定，孵化后早期发育阶段显著升高。受精后10天（dpf）时T3水平达到峰值，为0.28±0.01 ng g(-1)体重，21 dpf时T4达到峰值，为0.58±0.09 ng g(-1)体重。随后在后期发育过程中两种甲状腺激素均下降。本研究建立了斑马鱼甲状腺激素的基线，这对于理解甲状腺激素功能以及该物种未来甲状腺毒物的研究至关重要。
