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[用于检测尿液样本中嗜肺军团菌抗原的Binax酶免疫测定法和Biotest酶免疫测定法尿液抗原检测试剂盒的比较与评估]

[Comparison and evaluation of the Binax EIA and Biotest EIA urinary antigen kits for detection of Legionella pneumophila antigen in urine samples].

作者信息

Rastawicki Waldemar, Rokosz Natalia, Jagielski Marek

机构信息

Zakład Bakteriologii Narodowego Instytutu Zdrowia Publicznego-Państwowego Zakładu Higieny w Warszawie.

出版信息

Med Dosw Mikrobiol. 2011;63(4):315-20.

PMID:22384664
Abstract

The Binax and the Biotest urinary antigen kits for detection of L. pneumophila antigen were compared by testing of selected 67 urine samples obtained from EWGLI as reference samples in External Quality Assessment Scheme. Thirty nine were positive with the Binax kit (100% of sensitivity), and 33 were positive with the Biotest (84.6% of sensitivity). The test specificities were 100% for the both kits. It was concluded that the Binax kit was more suitable for the routine diagnosis of Legionella infections than the Biotest kit.

摘要

通过对从欧洲军团病监测网络(EWGLI)获取的67份尿液样本进行检测,将用于检测嗜肺军团菌抗原的Binax和Biotest尿液抗原检测试剂盒作为外部质量评估计划中的参考样本进行了比较。Binax试剂盒检测出39份呈阳性(灵敏度为100%),Biotest试剂盒检测出33份呈阳性(灵敏度为84.6%)。两种试剂盒的检测特异性均为100%。得出的结论是,与Biotest试剂盒相比,Binax试剂盒更适合用于军团菌感染的常规诊断。

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Med Dosw Mikrobiol. 2011;63(4):315-20.
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