用于检测嗜肺军团菌血清1型抗原的Binax军团菌尿抗原酶免疫分析试剂盒与Binax放射免疫分析尿抗原试剂盒的比较。
Comparison of Binax Legionella Urinary Antigen EIA kit with Binax RIA Urinary Antigen kit for detection of Legionella pneumophila serogroup 1 antigen.
作者信息
Hackman B A, Plouffe J F, Benson R F, Fields B S, Breiman R F
机构信息
Ohio State University Medical Center, Columbus 43210, USA.
出版信息
J Clin Microbiol. 1996 Jun;34(6):1579-80. doi: 10.1128/jcm.34.6.1579-1580.1996.
Abstract
The Legionella Urinary Antigen EIA kit (Binax, Portland, Maine) was compared with the EQUATE RIA Legionella Urinary Antigen kit (Binax) for its ability to detect the presence of urinary antigens to Legionella pneumophila serogroup 1. Urine specimens from patients without Legionnaires' disease (n = 33) were negative by both methods (specificity, 100%). Twenty (77%) of 26 urine specimens from patients with Legionnaires' disease positive by the radioimmunoassay kit were also positive by the enzyme immunoassay (EIA) kit. If the cutoff for a positive EIA result were lowered to a ration of > or = 2.5, 23 of 26 (88%) urine specimens would have been positive by EIA and the specificity would remain 100%. Use of the EIA kit is an acceptable method for detecting L. pneumophila serogroup 1 urinary antigens by laboratories that do not want to handle radioactive materials.
摘要
将军团菌尿抗原酶免疫分析试剂盒（Binax，缅因州波特兰）与EQUATE放射免疫分析军团菌尿抗原试剂盒（Binax）检测嗜肺军团菌血清1型尿抗原的能力进行了比较。来自非军团病患者的尿液标本（n = 33）两种方法检测结果均为阴性（特异性为100%）。放射免疫分析试剂盒检测为阳性的26例军团病患者尿液标本中，20例（77%）酶免疫分析（EIA）试剂盒检测也呈阳性。如果将EIA阳性结果的临界值降至≥2.5，则26例尿液标本中有23例（88%）EIA检测呈阳性，且特异性仍为100%。对于不想处理放射性物质的实验室，使用EIA试剂盒是检测嗜肺军团菌血清1型尿抗原的一种可接受方法。
相似文献
1
Comparison of Binax Legionella Urinary Antigen EIA kit with Binax RIA Urinary Antigen kit for detection of Legionella pneumophila serogroup 1 antigen.用于检测嗜肺军团菌血清1型抗原的Binax军团菌尿抗原酶免疫分析试剂盒与Binax放射免疫分析尿抗原试剂盒的比较。
J Clin Microbiol. 1996 Jun;34(6):1579-80. doi: 10.1128/jcm.34.6.1579-1580.1996.
2
Detection of Legionella pneumophila antigen in urine samples by the BinaxNOW immunochromatographic assay and comparison with both Binax Legionella Urinary Enzyme Immunoassay (EIA) and Biotest Legionella Urin Antigen EIA.采用BinaxNOW免疫层析法检测尿液样本中嗜肺军团菌抗原，并与Binax军团菌尿液酶免疫测定法（EIA）和Biotest军团菌尿液抗原EIA进行比较。
J Med Microbiol. 2001 Jun;50(6):509-516. doi: 10.1099/0022-1317-50-6-509.
3
Comparison of the Binax Legionella urinary antigen enzyme immunoassay (EIA) with the Biotest Legionella Urin antigen EIA for detection of Legionella antigen in both concentrated and nonconcentrated urine samples.将Binax军团菌尿抗原酶免疫测定法（EIA）与Biotest军团菌尿抗原EIA进行比较，以检测浓缩和未浓缩尿液样本中的军团菌抗原。
J Clin Microbiol. 1998 Sep;36(9):2718-22. doi: 10.1128/JCM.36.9.2718-2722.1998.
4
Assessment of a new test to detect Legionella urinary antigen for the diagnosis of Legionnaires' Disease.评估一种用于检测军团菌尿抗原以诊断军团病的新检测方法。
Diagn Microbiol Infect Dis. 2001 Dec;41(4):199-203. doi: 10.1016/s0732-8893(01)00308-x.
5
Comparison of radioimmunoassay and enzyme immunoassay kits for detection of Legionella pneumophila serogroup 1 antigen in both concentrated and nonconcentrated urine samples.用于检测浓缩和非浓缩尿液样本中嗜肺军团菌1型抗原的放射免疫分析试剂盒和酶免疫分析试剂盒的比较
J Clin Microbiol. 1997 Jun;35(6):1627-9. doi: 10.1128/jcm.35.6.1627-1629.1997.
6
Evaluation of a rapid immunochromatographic assay for the detection of Legionella antigen in urine samples.用于检测尿液样本中军团菌抗原的快速免疫层析测定法的评估。
Eur J Clin Microbiol Infect Dis. 1999 Dec;18(12):896-8. doi: 10.1007/s100960050427.
7
Rapid diagnosis of Legionella pneumophila serogroup 1 infection with the Binax enzyme immunoassay urinary antigen test.采用Binax酶免疫法尿液抗原检测快速诊断嗜肺军团菌1型感染
J Clin Microbiol. 1997 Apr;35(4):954-6. doi: 10.1128/jcm.35.4.954-956.1997.
8
[Evaluation of a new ELISA (Bartels) for detection of Legionella pneumophila antigen in urine].[评估一种用于检测尿液中嗜肺军团菌抗原的新型酶联免疫吸附测定法（巴特尔氏法）]
Enferm Infecc Microbiol Clin. 2002 Mar;20(3):106-9. doi: 10.1016/s0213-005x(02)72758-4.
9
[Evaluation of urinary antigen detection methods for rapid diagnosis of Legionella pneumonia].[用于军团菌肺炎快速诊断的尿抗原检测方法评估]
Kansenshogaku Zasshi. 1999 May;73(5):421-8. doi: 10.11150/kansenshogakuzasshi1970.73.421.
10
Evaluation of the Oxoid Xpect Legionella test kit for detection of Legionella pneumophila serogroup 1 antigen in urine.用于检测尿液中嗜肺军团菌血清1型抗原的奥克托克斯Xpect军团菌检测试剂盒评估
J Clin Microbiol. 2009 Jul;47(7):2272-4. doi: 10.1128/JCM.00397-09. Epub 2009 May 6.
引用本文的文献
1
Detection of Legionella antigen in nonconcentrated and concentrated urine samples by a new immunochromatographic assay.通过一种新型免疫层析检测法检测非浓缩和浓缩尿液样本中的嗜肺军团菌抗原。
Eur J Clin Microbiol Infect Dis. 2008 Dec;27(12):1249-51. doi: 10.1007/s10096-008-0559-6. Epub 2008 Jun 27.
2
Clinical utility of a Legionella pneumophila urinary antigen test in a large university teaching hospital.嗜肺军团菌尿抗原检测在大型大学教学医院中的临床应用
Can J Infect Dis. 2003 Mar;14(2):85-8. doi: 10.1155/2003/642159.
3
[Definition of low respiratory tract infections].[下呼吸道感染的定义]
Med Mal Infect. 2006 Nov-Dec;36(11-12):538-45. doi: 10.1016/j.medmal.2006.05.014. Epub 2006 Jul 11.
4
Sensitivity of urinary antigen test in relation to clinical severity in a large outbreak of Legionella pneumonia in Spain.西班牙军团菌肺炎大规模暴发中尿抗原检测敏感性与临床严重程度的关系
Eur J Clin Microbiol Infect Dis. 2005 Jul;24(7):488-91. doi: 10.1007/s10096-005-1361-3.
5
Legionella and Legionnaires' disease: 25 years of investigation.军团菌与军团病：25年的研究历程
Clin Microbiol Rev. 2002 Jul;15(3):506-26. doi: 10.1128/CMR.15.3.506-526.2002.
6
Polymerase chain reaction for detection of Legionellae DNA in urine samples from patients with community-acquired pneumonia.聚合酶链反应检测社区获得性肺炎患者尿液样本中的军团菌DNA。
Folia Microbiol (Praha). 2000;45(5):469-72. doi: 10.1007/BF02817623.
7
Evaluation of the Binax and Biotest urinary antigen kits for detection of Legionnaires' disease due to multiple serogroups and species of Legionella.评估Binax和Biotest尿抗原检测试剂盒用于检测由多种血清群和军团菌属物种引起的军团病。
J Clin Microbiol. 2000 Jul;38(7):2763-5. doi: 10.1128/JCM.38.7.2763-2765.2000.
8
Comparison of the Binax Legionella urinary antigen enzyme immunoassay (EIA) with the Biotest Legionella Urin antigen EIA for detection of Legionella antigen in both concentrated and nonconcentrated urine samples.将Binax军团菌尿抗原酶免疫测定法（EIA）与Biotest军团菌尿抗原EIA进行比较，以检测浓缩和未浓缩尿液样本中的军团菌抗原。
J Clin Microbiol. 1998 Sep;36(9):2718-22. doi: 10.1128/JCM.36.9.2718-2722.1998.
9
Comparison of radioimmunoassay and enzyme immunoassay kits for detection of Legionella pneumophila serogroup 1 antigen in both concentrated and nonconcentrated urine samples.用于检测浓缩和非浓缩尿液样本中嗜肺军团菌1型抗原的放射免疫分析试剂盒和酶免疫分析试剂盒的比较
J Clin Microbiol. 1997 Jun;35(6):1627-9. doi: 10.1128/jcm.35.6.1627-1629.1997.
10
Rapid diagnosis of Legionella pneumophila serogroup 1 infection with the Binax enzyme immunoassay urinary antigen test.采用Binax酶免疫法尿液抗原检测快速诊断嗜肺军团菌1型感染
J Clin Microbiol. 1997 Apr;35(4):954-6. doi: 10.1128/jcm.35.4.954-956.1997.
本文引用的文献
1
Surveillance for Legionnaires' disease. Risk factors for morbidity and mortality.军团病监测。发病和死亡的危险因素。
Arch Intern Med. 1994 Nov 14;154(21):2417-22.
2
Reevaluation of the definition of Legionnaires' disease: use of the urinary antigen assay. Community Based Pneumonia Incidence Study Group.军团病定义的重新评估：尿抗原检测的应用。社区肺炎发病率研究组。
Clin Infect Dis. 1995 May;20(5):1286-91. doi: 10.1093/clinids/20.5.1286.
3
Rapid radioimmunoassay diagnosis of Legionnaires' disease: detection and partial characterization of urinary antigen.军团病的快速放射免疫测定诊断：尿抗原的检测与部分特性分析
Ann Intern Med. 1981 May;94(5):601-5. doi: 10.7326/0003-4819-94-5-601.
4
Legionella pneumonia in the United States: the distribution of serogroups and species causing human illness.美国军团菌肺炎：导致人类疾病的血清群和菌种分布
J Infect Dis. 1984 May;149(5):819. doi: 10.1093/infdis/149.5.819.
5
Reactivity of serum from patients with suspected legionellosis against 29 antigens of legionellaceae and Legionella-like organisms by indirect immunofluorescence assay.采用间接免疫荧光法检测疑似军团病患者血清对军团菌科及类军团菌29种抗原的反应性。
J Infect Dis. 1983 Jan;147(1):23-31. doi: 10.1093/infdis/147.1.23.
6
Retrospective evaluation of the Du Pont radioimmunoassay kit for detection of Legionella pneumophila serogroup 1 antigenuria in humans.对杜邦放射免疫分析试剂盒用于检测人类嗜肺军团菌血清1型抗原尿的回顾性评估。
J Clin Microbiol. 1988 Sep;26(9):1775-8. doi: 10.1128/jcm.26.9.1775-1778.1988.
7
Legionnaires' disease: isolation of a bacterium and demonstration of its role in other respiratory disease.军团病：一种细菌的分离及其在其他呼吸道疾病中的作用证明
N Engl J Med. 1977 Dec 1;297(22):1197-203. doi: 10.1056/NEJM197712012972202.
Zotero 插件