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三种检测方法(巴特尔酶免疫测定法[EIA]、比奥泰斯特酶免疫测定法和宾纳克斯即时免疫层析试验)检测尿液中嗜肺军团菌血清1型抗原的诊断敏感性比较。

Comparison of diagnostic sensitivities of three assays (Bartels enzyme immunoassay [EIA], Biotest EIA, and Binax NOW immunochromatographic test) for detection of Legionella pneumophila serogroup 1 antigen in urine.

作者信息

Guerrero Carmen, Toldos Carmen M, Yagüe Genoveva, Ramírez Cristobal, Rodríguez Tomás, Segovia Manuel

机构信息

Servicio de Microbiología, Hospital "J M Morales Meseguer," 30008 Murcia, Spain.

出版信息

J Clin Microbiol. 2004 Jan;42(1):467-8. doi: 10.1128/JCM.42.1.467-468.2004.

DOI:10.1128/JCM.42.1.467-468.2004
PMID:14715807
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321653/
Abstract

The Bartels enzyme immunoassay (EIA), Biotest EIA, and Binax NOW immunochromatographic test (ICT) urinary antigen kits for the detection of Legionella pneumophila serogroup 1 were compared using 178 frozen urine samples. When nonconcentrated urine samples were used, the sensitivity levels of both enzyme EIAs were significantly higher than the sensitivity level of the ICT (Bartels EIA, 71.3%; Biotest EIA, 65.1%; Binax NOW ICT, 37% [P < 0.001]). After concentration of the urine samples, no significant differences in sensitivity were found among the three tests.

摘要

使用178份冷冻尿液样本,对用于检测嗜肺军团菌血清1型的巴特尔酶免疫测定法(EIA)、比奥泰斯特EIA和Binax NOW免疫层析试验(ICT)尿液抗原检测试剂盒进行了比较。当使用未浓缩的尿液样本时,两种酶免疫测定法的灵敏度水平均显著高于免疫层析试验的灵敏度水平(巴特尔EIA为71.3%;比奥泰斯特EIA为65.1%;Binax NOW免疫层析试验为37%[P<0.001])。尿液样本浓缩后,三种检测方法在灵敏度上未发现显著差异。

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[The role of the clinical microbiology laboratory during the outbreak of Legionella spp. in the municipality of Alcoy: the effectiveness of the different diagnosis methods].[阿利坎特市军团菌属爆发期间临床微生物实验室的作用:不同诊断方法的有效性]
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The impact of diagnosis by legionella urinary antigen test on the epidemiology and outcomes of Legionnaires' disease.军团菌尿抗原检测诊断对军团病流行病学及预后的影响。
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