Franzin L, Cabodi D
Infectious Diseases Unit, University of Turin, Italy.
New Microbiol. 2000 Oct;23(4):383-9.
We evaluated two commercial enzyme immunoassay kits, Binax EIA (for detection of soluble antigen of Legionella pneumophila serogroup 1) and Biotest EIA (for detection of antigens of Legionella pneumophila serogroups and other Legionella spp.) in order to introduce this test routinely for the diagnosis of Legionnaires' disease (LD) in our Laboratory. Frozen non-concentrated urine samples belonging to 45 patients with and without LD were tested. The sensitivity of Binax EIA and Biotest EIA was 47.4% and 42.1% respectively, the specificity was 95% by both tests. Biotest did not detect antigen from a patient with culture-proven infection of L. pneumophila serogroup 6. The detection of urinary antigen by both EIA tests is a useful tool for rapid diagnosis of LD, especially when samples are unavailable for culture; the sensitivity may be increased if the assay is performed on unfrozen and concentrated samples.
我们评估了两种商用酶免疫分析试剂盒，即Binax EIA（用于检测嗜肺军团菌血清1型可溶性抗原）和Biotest EIA（用于检测嗜肺军团菌血清型及其他军团菌属的抗原），以便在我们实验室将该检测常规用于军团病（LD）的诊断。对45例患有和未患LD的患者的冷冻非浓缩尿液样本进行了检测。Binax EIA和Biotest EIA的灵敏度分别为47.4%和42.1%，两种检测的特异性均为95%。Biotest未检测到一名经培养证实感染嗜肺军团菌血清6型患者的抗原。两种酶免疫分析检测尿液抗原对于LD的快速诊断是一种有用的工具，尤其是在无法获得用于培养的样本时；如果对未冷冻和浓缩的样本进行检测，灵敏度可能会提高。
