Department of Ecology, University of Calabria, Rende, Italy.
J Histochem Cytochem. 2012 May;60(5):359-65. doi: 10.1369/0022155412439717. Epub 2012 Mar 2.
The ubiquitin-conjugating enzyme (UbcH10) plays important roles in the regulation of cell cycle progression. Recently, UbcH10 expression has been demonstrated in several human and experimental tumors, and proteasome inhibitors have been tested in trials for pulmonary neoplasms; however, the underlying mechanisms as well as the clinicopathological relevance of UbcH10 in the genesis and progression of lung cancer remain largely unknown. Therefore, the authors evaluated the expression of UbcH10 in human lung cancer and evaluated its possible diagnostic and prognostic use. They found that most cases of lung adenocarcinoma, squamous cell carcinoma, and large cell and small cell carcinoma were positive for UbcH10. The expression levels of UbcH10 progressively increased with decreasing degree of tumor differentiation. There was a statistically significant difference of UbcH10 positivity between grade I/III of lung adenocarcinoma (p=0.013) and squamous cell carcinoma (p=0.002). No significant differences were found between histological types (p=0.072). In the case of cell blocks prepared from pleural effusions, inflammatory and reactive mesothelial elements did not show appreciable UbcH10 expression, whereas neoplastic cells exhibited clear UbcH10 positivity. The results suggest that UbcH10 might represent a new and promising diagnostic and prognostic marker in both histologic and cytologic specimens of lung cancer.
泛素结合酶（UbcH10）在细胞周期进程的调控中发挥着重要作用。最近，在几种人类和实验性肿瘤中已经证明了 UbcH10 的表达，并且蛋白酶体抑制剂已在肺肿瘤的试验中进行了测试；然而，UbcH10 在肺癌的发生和发展中的潜在机制及其与临床病理的相关性仍在很大程度上未知。因此，作者评估了 UbcH10 在人类肺癌中的表达，并评估了其在诊断和预后中的可能用途。他们发现，大多数肺腺癌、鳞状细胞癌、大细胞癌和小细胞癌的病例均为 UbcH10 阳性。UbcH10 的表达水平随肿瘤分化程度的降低而逐渐升高。肺腺癌（p=0.013）和鳞状细胞癌（p=0.002）的 I/III 级之间 UbcH10 阳性率存在统计学显著差异。不同组织学类型之间无显著差异（p=0.072）。在胸腔积液制备的细胞块中，炎症和反应性间皮细胞没有明显的 UbcH10 表达，而肿瘤细胞则表现出明显的 UbcH10 阳性。结果表明，UbcH10 可能是肺癌组织学和细胞学标本中一种新的有前途的诊断和预后标志物。
