Hossain Rajib, Kim Kyung-Il, Li Xin, Lee Hyun Jae, Lee Choong Jae
Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea.
Brain Korea 21 FOUR Project for Medical Science, Chungnam National University, Daejeon 35015, Republic of Korea.
Biomol Ther (Seoul). 2022 Sep 1;30(5):473-478. doi: 10.4062/biomolther.2022.088. Epub 2022 Aug 22.
In this study, we examined whether engeletin exerts an effect on the gene expression of MUC5AC mucin, in human pulmonary epithelial NCI-H292 cells. The cells were pretreated with engeletin for 30 min and stimulated with phorbol 12-myristate 13-acetate (PMA), for the following 24 h. The effect of engeletin on PMA-induced nuclear factor kappa B (NF-kB) signaling pathway was also investigated. Engeletin suppressed the mRNA expression and production of MUC5AC mucin, induced by PMA through the inhibition of degradation of inhibitory kappa Bα (IkBα) and NF-kB p65 nuclear translocation. These results suggest engeletin inhibits the gene expression of mucin through regulation of NF-kB signaling pathway, in human airway epithelial cells.
在本研究中，我们检测了恩格letin对人肺上皮NCI-H292细胞中MUC5AC粘蛋白基因表达的影响。细胞先用恩格letin预处理30分钟，然后用佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）刺激24小时。还研究了恩格letin对PMA诱导的核因子κB（NF-κB）信号通路的影响。恩格letin通过抑制抑制性κBα（IkBα）的降解和NF-κB p65核转位，抑制了PMA诱导的MUC5AC粘蛋白的mRNA表达和产生。这些结果表明，恩格letin通过调节NF-κB信号通路抑制人气道上皮细胞中粘蛋白的基因表达。
