Yun Chawon, Lee Hyun Jae, Lee Choong Jae
Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea.
Smith Liberal Arts College and Department of Addiction Science, Graduate School, Sahmyook University, Seoul 01795, Republic of Korea.
Biomol Ther (Seoul). 2021 Nov 1;29(6):637-642. doi: 10.4062/biomolther.2021.091.
In this study, we investigated whether eriodictyol exerts an effect on the production and gene expression of MUC5AC mucin in human pulmonary epithelial NCI-H292 cells. The cells were pretreated with eriodictyol for 30 min and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 h. The effect of eriodictyol on PMA-induced nuclear factor kappa B (NF-κB) signaling pathway was also investigated. Eriodictyol suppressed the MUC5AC mucin production and gene expression induced by PMA via suppression of inhibitory kappa Bα degradation and NF-κB p65 nuclear translocation. These results suggest that eriodictyol inhibits mucin gene expression and production in human airway epithelial cells via regulation of the NF-κB signaling pathway.
在本研究中，我们调查了圣草酚是否对人肺上皮NCI-H292细胞中MUC5AC粘蛋白的产生和基因表达有影响。细胞先用圣草酚预处理30分钟，然后用佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）刺激24小时。还研究了圣草酚对PMA诱导的核因子κB（NF-κB）信号通路的影响。圣草酚通过抑制抑制性κBα降解和NF-κB p65核转位，抑制了PMA诱导的MUC5AC粘蛋白产生和基因表达。这些结果表明，圣草酚通过调节NF-κB信号通路抑制人气道上皮细胞中粘蛋白基因的表达和产生。
