Khon-Kaen University, Khon-Kaen 40002, Thailand.
Phytochemistry. 2012 May;77:60-9. doi: 10.1016/j.phytochem.2012.02.002. Epub 2012 Mar 3.
The cDNA of a benzophenone synthase (BPS), a type III polyketide synthase (PKS), was cloned and the recombinant protein expressed from the fruit pericarps of Garcinia mangostana L., which contains mainly prenylated xanthones. The obtained GmBPS showed an amino acid sequence identity of 77-78% with other plant BPSs belonging to the same family (Clusiaceae). The recombinant enzyme produced 2,4,6-trihydroxybenzophenone as the predominant product with benzoyl CoA as substrate. It also accepted other substrates, such as other plant PKSs, and used 1-3 molecules of malonyl CoA to form various phloroglucinol-type and polyketide lactone-type compounds. Thus, providing GmBPS with various substrates in vivo might redirect the xanthone biosynthetic pathway.
从含有主要被prenylated 的黄烷酮的藤黄果皮中克隆得到了苯甲酮合酶（BPS）的 cDNA，该酶是一种 III 型聚酮合酶（PKS）。所得到的 GmBPS 与其他属于同一科（藤黄科）的植物 BPS 具有 77-78%的氨基酸序列同一性。该重组酶以苯甲酰辅酶 A 为底物生成 2,4,6-三羟基二苯甲酮作为主要产物。它还接受其他底物，如其他植物 PKS，并使用 1-3 个丙二酰辅酶 A 分子形成各种间苯三酚型和聚酮内酯型化合物。因此，在体内为 GmBPS 提供各种底物可能会使黄烷酮生物合成途径发生重定向。
