Department of Botany, Saifia Sciences Postgraduate College, Bhopal, Madhya Pradesh, India.
Bull Environ Contam Toxicol. 2012 May;88(5):659-65. doi: 10.1007/s00128-012-0570-6. Epub 2012 Mar 4.
In the present study effects of herbicides glyphosate (GP), alachlor (AL) and maleic hydrazide (MH) is studied on mitotic cells of Trigonella foenum-graecum L. Seeds of T. foenum-graecum L. treated with a series of concentrations ranging from 0.1%, 0.2%, 0.3%, 0.4% and 0.5% for 1, 2 and 6 h and their effect on mitotic index and chromosomal aberrations was studied. The results indicate that these herbicides reduced mitotic index in dose-dependent manner. In addition, increase in the percentage of abnormal mitotic plates was observed in herbicide treated groups which was both concentration and time dependent. Commonly observed abnormalities were c-mitosis, laggards, bridges, stickiness, c-anaphase, precocious separation, un-equal distribution and fragments. The result of the present investigation indicates that commonly used herbicides GP, AL and MH have significant genotoxic effect on T. foenum-graecum plant.
本研究研究了除草剂草甘膦（GP）、甲草胺（AL）和马来酰肼（MH）对苦豆子 Trigonella foenum-graecum L. 种子有丝分裂细胞的影响。用一系列浓度（0.1%、0.2%、0.3%、0.4%和 0.5%）处理苦豆子种子 1、2 和 6 小时，研究其对有丝分裂指数和染色体畸变的影响。结果表明，这些除草剂以剂量依赖的方式降低了有丝分裂指数。此外，在除草剂处理组中观察到异常有丝分裂板的百分比增加，这是浓度和时间依赖性的。常见的异常现象有 c-有丝分裂、滞后、桥、粘性、c-后期、早熟分离、不均匀分布和片段。本研究结果表明，常用的除草剂草甘膦、甲草胺和马来酰肼对苦豆子植物具有显著的遗传毒性。
