Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, University of Santiago de Compostela, 27002 Lugo, Spain.
Environ Sci Pollut Res Int. 2012 Sep;19(8):3235-49. doi: 10.1007/s11356-012-0830-3. Epub 2012 Mar 4.
The use of veterinary drugs in food production focuses on the control and improvement of animal health. The disadvantage of this practice is that pharmaceuticals and their metabolites are released into the environment, finding their way to natural water systems and becoming a potential risk to non-target organism.
This paper reports the development and validation of a quantitative method, based on high-performance liquid chromatography coupled to tandem mass spectrometry, for the simultaneous analysis of 21 veterinary drugs, antimicrobials, corticosteroids, coccidiostats and antifungal agents, in surface water.
The precision of the method was established by calculating the mean recoveries, which were in the range of 94-101%. The developed method was employed to conduct the first monitoring study on the presence of veterinary drugs in the Galicia region, Northwest of Spain and was applied to 235 surface water samples. Eleven veterinary drugs were detected at concentrations from below the limit of quantification to 2,978.6 ng L(-1). Limits of detection and quantification were in the range of 6.2 (betamethasone, cortisone, decoquinate, dexamethasone, maduramycin, monensin, narasin, salinomycin, sulfachloropyridazine, sulfamethoxypyridazine and trimethoprim) to 12.5 ng L(-1) (for the rest of the selected drugs) and 12.5 (betamethasone, cortisone, decoquinate, dexamethasone, maduramycin, monensin, narasin, salinomycin, sulfachloropyridazine, sulfamethoxypyridazine and trimethoprim) to 25.0 ng L(-1) (for the remaining pharmaceuticals), respectively.
Sulfonamides were the group most frequently found, which are widely used in veterinary medicine.
在食品生产中使用兽药主要侧重于控制和改善动物健康。这种做法的缺点是，药物及其代谢物会释放到环境中，进入自然水系，对非目标生物构成潜在风险。
本文报告了一种基于高效液相色谱-串联质谱法的定量方法的开发和验证，用于同时分析地表水 21 种兽药、抗生素、皮质甾类、球虫抑制剂和抗真菌剂。
该方法的精密度通过计算回收率来确定，回收率范围为 94-101%。该方法已用于对西班牙西北部加利西亚地区存在兽药的首次监测研究，并应用于 235 个地表水样本。在浓度低于定量下限至 2978.6ng/L 的情况下，检测到 11 种兽药。检测限和定量限范围为 6.2（倍他米松、可的松、癸氧喹酯、地塞米松、莫能菌素、那拉菌素、那拉霉素、盐霉素、磺胺氯哒嗪、磺胺二甲嘧啶和甲氧苄啶）至 12.5ng/L（其余选定药物）和 12.5（倍他米松、可的松、癸氧喹酯、地塞米松、莫能菌素、那拉菌素、那拉霉素、盐霉素、磺胺氯哒嗪、磺胺二甲嘧啶和甲氧苄啶）至 25.0ng/L（其余药物）。
磺胺类药物是最常发现的一组药物，它们在兽医中广泛使用。
