Mitsui H
Department of Urology, Yamaguchi University School of Medicine.
Hinyokika Kiyo. 1990 Jul;36(7):749-55.
We evaluated the effect of various immunosuppressive drugs in the SRC assay. We chose the most appropriate day to obtain the results of the SRC assay using the MBT-2 tumor. MBT-2 tumor-bearing mice of homogenic and heterogenic strains were separately given one of the three: cyclophosphamide, azathioprine or mizoribine. These drugs were evaluated on the 6th and the 11th day for the immunosuppressive effects expected from a host versus graft reaction. Concerning the homogenic mice that were injected with drugs, on the 11th day, the growth of the tumor was favorably suppressed in comparison with the growth of the control group (A-1) due to the anti-tumor effect of immunosuppressive drugs. We concluded that the 6th day was the most, appropriate day to obtain the results of this assay. Concerning the heterogenic mice, similar results were observed. In the mizoribine-group, we subcutaneously injected 200 mg/kg on alternate days. Yet the implanted tumor grew almost at the same rate as that of the A-1 group. In this group, a histological study showed reduction of the tumor cells and few inflammatory cells. We concluded that this drug was more useful than the others in the SRC assay and the suitable day for judgement was the 6th day.
我们在SRC试验中评估了各种免疫抑制药物的效果。我们选择了最合适的日子来获取使用MBT - 2肿瘤进行SRC试验的结果。将同基因和异基因品系的携带MBT - 2肿瘤的小鼠分别给予以下三种药物之一：环磷酰胺、硫唑嘌呤或米唑嘌呤。在第6天和第11天评估这些药物对于宿主与移植物反应所预期的免疫抑制效果。对于注射了药物的同基因小鼠，在第11天，由于免疫抑制药物的抗肿瘤作用，与对照组（A - 1）相比，肿瘤生长受到了明显抑制。我们得出结论，第6天是获得该试验结果的最合适日子。对于异基因小鼠，观察到了类似的结果。在米唑嘌呤组中，我们每隔一天皮下注射200 mg/kg。然而，植入的肿瘤生长速度几乎与A - 1组相同。在该组中，组织学研究显示肿瘤细胞减少且炎症细胞很少。我们得出结论，在SRC试验中这种药物比其他药物更有用，判断的合适日子是第6天。
