Department of Medical Biochemistry and Molecular Biology, Research Group of Predictive Diagnostics, Ernst Moritz Arndt University of Greifswald, Greifswalder Str. 11c, 17495 Karlsburg, Germany.
J Mater Sci Mater Med. 2012 May;23(5):1299-307. doi: 10.1007/s10856-012-4600-z. Epub 2012 Mar 9.
Surface modification of Titanium (Ti) by low-temperature plasma influences cell-material interactions. Therefore, this study aimed at examining serum cytokine levels and associations after intramuscular implantation (n = 8 rats/group) of Ti-plates with Plasma Polymerized Allyl Amine (Ti-PPAAm), Plasma Polymerized Acrylic Acid (Ti-PPAAc), and without such layers (Ti-Controls). Pro-inflammatory (IL-2, IFNγ, IL-6) and anti-inflammatory (IL-4, IL-10, IL-13) cytokines were measured weekly for 56 days. Ti-PPAAm caused increased IL-2 (d7-14, d35), increased IFNγ (d35) and decreased IL-10 (d35, d49-56). Ti-PPAAc induced divergent anti-inflammatory cytokine changes with increased IL-4 (d28-56) and decreased IL-10 (d42-56). Ti-Controls elicited increased IL-2 (d42) and IFNγ (d35-42, d56). IL-6 was not detected and IL-13 only in three samples, thus they do not influence the response against these Ti implants. Correlation analysis revealed surface-dependent associations between cytokines indicating the involvement of different inflammatory cell populations. Concluding, different plasma modifications induce specific serum cytokine profiles and associations indicating distinct inflammatory responses.
低温等离子体对钛（Ti）的表面改性会影响细胞与材料的相互作用。因此，本研究旨在研究肌肉内植入（每组 8 只大鼠）等离子聚合丙烯胺（Ti-PPAAm）、等离子聚合丙烯酸（Ti-PPAAc）和无此类层（Ti-Controls）的 Ti 板后血清细胞因子水平和相关性。每周测量促炎（IL-2、IFNγ、IL-6）和抗炎（IL-4、IL-10、IL-13）细胞因子 56 天。Ti-PPAAm 引起 IL-2（d7-14，d35）增加、IFNγ（d35）增加和 IL-10（d35、d49-56）减少。Ti-PPAAc 诱导抗炎细胞因子发生不同变化，IL-4（d28-56）增加，IL-10（d42-56）减少。Ti-Controls 引起 IL-2（d42）和 IFNγ（d35-42，d56）增加。未检测到 IL-6，仅在三个样本中检测到 IL-13，因此它们不会影响对这些 Ti 植入物的反应。相关分析显示细胞因子之间存在与表面相关的关联，表明涉及不同的炎症细胞群。结论：不同的等离子体改性会诱导特定的血清细胞因子谱和相关性，表明存在不同的炎症反应。
