Gabler Carolin, Zietz Carmen, Göhler Rebecca, Fritsche Andreas, Lindner Tobias, Haenle Maximilian, Finke Birgit, Meichsner Jürgen, Lenz Solvig, Frerich Bernhard, Lüthen Frank, Nebe J Barbara, Bader Rainer
Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, Doberaner Straße 142, 18057 Rostock, Germany.
Leibniz Institute for Plasma Science and Technology (INP e.V.) Greifswald, Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.
Int J Mol Sci. 2014 Feb 11;15(2):2454-64. doi: 10.3390/ijms15022454.
By means of plasma polymerization, positively charged, nanometre-thin coatings can be applied to implant surfaces. The aim of the present study was to quantify the adhesion of human bone cells in vitro and to evaluate the bone ongrowth in vivo, on titanium surfaces modified by plasma polymer coatings. Different implant surface configurations were examined: titanium alloy (Ti6Al4V) coated with plasma-polymerized allylamine (PPAAm) and plasma-polymerized ethylenediamine (PPEDA) versus uncoated. Shear stress on human osteoblast-like MG-63 cells was investigated in vitro using a spinning disc device. Furthermore, bone-to-implant contact (BIC) was evaluated in vivo. Custom-made conical titanium implants were inserted at the medial tibia of female Sprague-Dawley rats. After a follow-up of six weeks, the BIC was determined by means of histomorphometry. The quantification of cell adhesion showed a significantly higher shear stress for MG-63 cells on PPAAm and PPEDA compared to uncoated Ti6Al4V. Uncoated titanium alloyed implants showed the lowest BIC (40.4%). Implants with PPAAm coating revealed a clear but not significant increase of the BIC (58.5%) and implants with PPEDA a significantly increased BIC (63.7%). In conclusion, plasma polymer coatings demonstrate enhanced cell adhesion and bone ongrowth compared to uncoated titanium surfaces.
通过等离子体聚合，可以在植入物表面施加带正电荷的纳米级薄涂层。本研究的目的是在体外定量人骨细胞的黏附情况，并评估经等离子体聚合物涂层改性的钛表面在体内的骨生长情况。研究了不同的植入物表面构型：涂覆有等离子体聚合烯丙胺（PPAAm）和等离子体聚合乙二胺（PPEDA）的钛合金（Ti6Al4V）与未涂覆的钛合金。使用旋转盘装置在体外研究了人成骨样MG-63细胞上的剪切应力。此外，在体内评估了骨与植入物的接触（BIC）。将定制的锥形钛植入物插入雌性Sprague-Dawley大鼠的胫骨内侧。随访六周后，通过组织形态计量学确定BIC。细胞黏附定量显示，与未涂覆的Ti6Al4V相比，MG-63细胞在PPAAm和PPEDA上的剪切应力明显更高。未涂覆的钛合金植入物的BIC最低（40.4%）。涂覆PPAAm的植入物的BIC有明显但不显著的增加（58.5%），涂覆PPEDA的植入物的BIC显著增加（63.7%）。总之，与未涂覆的钛表面相比，等离子体聚合物涂层表现出增强的细胞黏附和骨生长。
