Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamilnadu, India.
Eur J Clin Microbiol Infect Dis. 2012 Sep;31(9):2311-7. doi: 10.1007/s10096-012-1570-5. Epub 2012 Mar 9.
We sought to analyse the presence of human papillomavirus (HPV), Chlamydia trachomatis and cytomegalovirus (CMV) infection in women with epithelial ovarian carcinomas. Polymerase chain reaction (PCR)-based detection of microbial infections was carried out. A total of 39 tissue samples were analysed with consensus and type-specific primers for HPV, primers specific for the cryptic plasmid of Chlamydia and primers for glycoprotein B of CMV. The samples analysed showed 40%, 80% and 50% positivity for HPV, Chlamydia and CMV infection, respectively, in cancerous ovarian tissues. The HPV type detected was HPV 6, with its genome integrated to the host genome in case of both invasive and borderline tumours and existed episomally in healthy controls. The patients with Chlamydia (odds ratio [OR] 32; 95% confidence interval [CI] 3.33, 307.65) and CMV infection (OR 8; 95% CI 0.888, 72.10) are at significantly higher risk of development of ovarian tumours. The present study validates the theory of chronic infections and inflammation in the pathogenesis of epithelial ovarian cancer. Further seroepidemiological studies and large fresh tissue sampling may represent the real prevalence of infections among ovarian carcinoma patients. This study is the first of its kind in detecting the bacterial and viral aetiologies in the development of ovarian carcinoma among Indian women.
我们旨在分析人乳头瘤病毒（HPV）、沙眼衣原体和巨细胞病毒（CMV）感染在患有上皮性卵巢癌的女性中的存在情况。采用聚合酶链反应（PCR）检测微生物感染。用 HPV 的通用和型特异性引物、沙眼衣原体隐蔽质粒的引物和 CMV 糖蛋白 B 的引物对 39 个组织样本进行了分析。分析的样本显示，HPV、沙眼衣原体和 CMV 感染在癌性卵巢组织中的阳性率分别为 40%、80%和 50%。检测到的 HPV 类型为 HPV 6，其基因组在侵袭性和交界性肿瘤中均整合到宿主基因组中，而在健康对照中则以游离体形式存在。患有沙眼衣原体（优势比[OR] 32；95%置信区间[CI] 3.33，307.65）和 CMV 感染（OR 8；95% CI 0.888，72.10）的患者发生卵巢肿瘤的风险显著增加。本研究验证了慢性感染和炎症在卵巢上皮性癌发病机制中的理论。进一步的血清流行病学研究和大量新鲜组织采样可能代表了卵巢癌患者感染的真实流行率。这是首次在印度女性中检测到细菌和病毒病因在卵巢癌发展中的作用。
