磷脂自组装的热力学。
Thermodynamics of phospholipid self-assembly.
机构信息
Max-Planck-Institut für biophysikalische Chemie, Göttingen, Germany.
出版信息
Biophys J. 2012 Mar 7;102(5):1079-87. doi: 10.1016/j.bpj.2012.01.049. Epub 2012 Mar 6.
Abstract
Negatively charged phospholipids are an important component of biological membranes. The thermodynamic parameters governing self-assembly of anionic phospholipids are deduced here from isothermal titration calorimetry. Heats of demicellization were determined for dioctanoyl phosphatidylglycerol (PG) and phosphatidylserine (PS) at different ionic strengths, and for dioctanoyl phosphatidic acid at different pH values. The large heat capacity (ΔC°(P) ∼ -400 J.mol(-1) K(-1) for PG and PS), and zero enthalpy at a characteristic temperature near the physiological range (T(∗) ~ 300 K for PG and PS), demonstrate that the driving force for self-assembly is the hydrophobic effect. The pH and ionic-strength dependences indicate that the principal electrostatic contribution to self-assembly comes from the entropy associated with the electrostatic double layer, in agreement with theoretical predictions. These measurements help define the thermodynamic effects of anionic lipids on biomembrane stability.
摘要
带负电荷的磷脂是生物膜的重要组成部分。本文通过等温滴定量热法推导了阴离子型磷脂自组装的热力学参数。在不同离子强度下测定了二油酰基磷脂酰甘油（PG）和磷脂酰丝氨酸（PS）的去胶束化热，在不同 pH 值下测定了二油酰基磷脂酸的去胶束化热。大的热容（ΔC°(P)∼-400 J·mol(-1)·K(-1)，对于 PG 和 PS），以及在接近生理范围的特征温度（T(∗)~300 K，对于 PG 和 PS）下的焓为零，证明了自组装的驱动力是疏水效应。pH 值和离子强度的依赖性表明，自组装的主要静电贡献来自于与静电双电层相关的熵，这与理论预测一致。这些测量有助于确定阴离子脂质对生物膜稳定性的热力学影响。
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