Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Belgium.
Arch Virol. 2012 Jun;157(6):1177-82. doi: 10.1007/s00705-012-1273-3. Epub 2012 Mar 20.
Indirect immunofluorescence techniques targeting the rotavirus (RV) protein VP6 are used to differentiate RV species. The ICTV recognizes RV species A to E and two tentative species, F and G. A potential new RV species, ADRV-N, has been described. Phylogenetic trees and pairwise identity frequency graphs were constructed with more than 400 available VP6 sequences and seven newly determined VP6 sequences of RVD strains. All RV species were separated into distinct phylogenetic clusters. An amino acid sequence cutoff value of 53% firmly permitted differentiation of RV species, and ADRV-N was tentatively assigned to a novel RV species H (RVH).
间接免疫荧光技术针对轮状病毒（RV）蛋白 VP6 用于区分 RV 种。国际病毒分类委员会（ICTV）承认 RV 种 A 到 E 和两个暂定种 F 和 G。已描述了一种潜在的新 RV 种，ADRV-N。用 400 多个可用的 VP6 序列和 7 个新测定的 RVD 株的 VP6 序列构建了系统进化树和成对身份频率图。所有 RV 种都被分离到不同的系统进化簇中。氨基酸序列截值为 53%，可明确区分 RV 种，ADRV-N 暂定为新的 RV 种 H（RVH）。
