Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea.
J Virol. 2012 May;86(10):5953-8. doi: 10.1128/JVI.07149-11. Epub 2012 Mar 21.
The rapid transmission of the pandemic 2009 H1N1 influenza virus (pH1N1) among humans has raised the concern of a potential emergence of reassortment between pH1N1 and highly pathogenic influenza strains, especially the avian H5N1 influenza virus. Here, we report that the cold-adapted pH1N1 live attenuated vaccine (CApH1N1) elicits cross-reactive immunity to seasonal and H5 influenza A viruses in the mouse model. Immunization with CApH1N1 induced both systemic and mucosal antibodies with broad reactivity to seasonal and H5 strains, including HAPI H5N1 and the avian H5N2 virus, providing complete protection against heterologous and heterosubtypic lethal challenges. Our results not only accentuate the merit of using live attenuated influenza virus vaccines in view of cross-reactivity but also represent the potential of CApH1N1 live vaccine for mitigating the clinical severity of infections that arise from reassortments between pH1N1 and highly pathogenic H5 subtype viruses.
2009 年甲型 H1N1 流感病毒（pH1N1）在人类中的快速传播引起了人们对 pH1N1 与高致病性流感株（尤其是禽流感 H5N1 病毒）之间可能发生重组的关注。在这里，我们报告称，冷适应 pH1N1 活减毒疫苗（CApH1N1）在小鼠模型中引发针对季节性和 H5 流感 A 病毒的交叉反应性免疫。用 CApH1N1 免疫可诱导全身性和黏膜性抗体，对季节性和 H5 株具有广泛的反应性，包括 HAPI H5N1 和禽流感 H5N2 病毒，可完全预防异源和异亚型致死性挑战。我们的研究结果不仅突出了使用活减毒流感病毒疫苗具有交叉反应性的优点，而且还表明 CApH1N1 活疫苗有可能减轻 pH1N1 与高致病性 H5 亚型病毒之间重组引起的感染的临床严重程度。
