Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
J Virol. 2011 Oct;85(20):10905-8. doi: 10.1128/JVI.00700-11. Epub 2011 Aug 17.
The conserved influenza virus hemagglutinin (HA) stem domain elicits cross-reactive antibodies, but epitopes in the globular head typically elicit strain-specific responses because of the hypervariability of this region. We isolated human monoclonal antibody 5J8, which neutralized a broad spectrum of 20th century H1N1 viruses and the 2009 pandemic H1N1 virus. Fine mapping of the interaction unexpectedly revealed a novel epitope between the receptor-binding pocket and the Ca₂ antigenic site on HA. This antibody exposes a new mechanism underlying broad immunity against H1N1 influenza viruses and identifies a conserved epitope that might be incorporated into engineered H1 virus vaccines.
保守的流感病毒血凝素（HA）茎域引发交叉反应性抗体，但由于该区域的高度变异性，球形头部的表位通常引发株特异性反应。我们分离出了人源单克隆抗体 5J8，该抗体中和了广泛的 20 世纪 H1N1 病毒和 2009 年大流行的 H1N1 病毒。出乎意料的是，对相互作用的精细作图揭示了 HA 上受体结合口袋和 Ca₂ 抗原位点之间的一个新表位。该抗体揭示了针对 H1N1 流感病毒产生广泛免疫力的新机制，并确定了一个可能被纳入工程 H1 病毒疫苗的保守表位。
