Department of Stomatology, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
Oral Dis. 2012 Oct;18(7):673-9. doi: 10.1111/j.1601-0825.2012.01924.x. Epub 2012 Mar 23.
The aim of this study was to investigate the relationship between podoplanin expression and proliferative activity of ameloblastomas and remnants of the odontogenic epithelium from dental follicles (DF) of unerupted teeth.
Thirty-three paraffin-embedded ameloblastomas and thirty-two DF obtained of unerupted teeth were analyzed by immunohistochemistry using anti-human podoplanin and anti-Ki-67 antibodies. Podoplanin expression in odontogenic epithelial cells was evaluated using a scoring method, and the Ki-67 labeling index was determined by the percentage of positive odontogenic cells.
All ameloblastomas displayed podoplanin expression in ameloblast-like cells of the epithelial islands. Membranous expression of podoplanin in ameloblastomas was stronger than in the remnants of odontogenic epithelium (P = 0.001). Statistically significant difference was observed between the cytoplasmic and membranous expression of podoplanin in the remnants of odontogenic epithelium (P = 0.001). The index of epithelial odontogenic proliferative activity, verified by Ki-67 expression, was higher in ameloblastomas vs remnants of odontogenic epithelium (P < 0.001). No statistically significant correlation was identified between podoplanin and the cellular odontogenic proliferative activity in meloblastomas and DF (P > 0.05).
These results provide evidence that there is no connection between podoplanin immunostaining and odontogenic cellular proliferative activity and suggest a role for membranous podoplanin expression in the local invasion of ameloblastomas.
本研究旨在探讨牙囊（DF）中未萌出牙的牙源性上皮细胞中 podoplanin 表达与成釉细胞瘤增殖活性之间的关系。
采用免疫组织化学方法，使用抗人 podoplanin 和抗 Ki-67 抗体分析 33 例石蜡包埋的成釉细胞瘤和 32 例未萌出牙的 DF。采用评分法评估牙源性上皮细胞中 podoplanin 的表达，并用阳性牙源性细胞的百分比来确定 Ki-67 标记指数。
所有成釉细胞瘤均在肿瘤上皮岛的成釉细胞样细胞中显示出 podoplanin 表达。成釉细胞瘤中 podoplanin 的膜表达强于牙源性上皮的残余物（P = 0.001）。牙源性上皮残余物中 podoplanin 的胞质和膜表达之间存在统计学显著差异（P = 0.001）。Ki-67 表达证实，成釉细胞瘤的上皮牙源性增殖活性指数高于牙源性上皮残余物（P < 0.001）。在成釉细胞瘤和 DF 中，podoplanin 与细胞牙源性增殖活性之间未发现统计学显著相关性（P > 0.05）。
这些结果表明，podoplanin 免疫染色与牙源性细胞增殖活性之间没有联系，并提示膜性 podoplanin 表达在成釉细胞瘤的局部浸润中起作用。
