Potificia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
J Breath Res. 2012 Jun;6(2):026001. doi: 10.1088/1752-7155/6/2/026001. Epub 2012 Mar 27.
The collection of exhaled breath condensates (EBC) is a noninvasive method for obtaining samples from the lungs. Eicosanoids are lipid mediators implicated in the asthmatic inflammatory response. The objective of our study was to investigate whether the profile of eicosanoid lipid mediators in EBC can characterize the inflammation in asthma and chronic obstructive pulmonary disease (COPD). EBC samples were collected from 22 healthy controls (C), 25 mild intermittent asthmatics (MIA), 20 with moderate to severe asthma (MSA) and 20 with moderate to severe COPD. EBC samples were analyzed by unique tandem mass spectrometry that allows the quantification of up to 25 eicosanoid mediators simultaneously. No differences were found between MIA and C. Subjects with MSA and COPD had higher levels of 6-keto, PGE2, LTB4, 11-12 EET and AA, while lower levels of LXA4, 11DHyTxB2, 11HETE and 8,9EET, when compared to MSA and C (p < 0.05). Our study shows that the analysis of EBC through mass spectrometry is mixed and has a similar response in MSA and COPD when compared to MIA and controls.
呼出气冷凝物（EBC）的收集是一种从肺部获取样本的非侵入性方法。二十碳烷类是参与哮喘炎症反应的脂类介质。我们的研究目的是探讨 EBC 中二十碳烷类脂类介质的特征是否可以表征哮喘和慢性阻塞性肺疾病（COPD）中的炎症。从 22 名健康对照者（C）、25 名轻度间歇性哮喘患者（MIA）、20 名中重度哮喘患者（MSA）和 20 名中重度 COPD 患者中收集 EBC 样本。EBC 样本通过独特的串联质谱分析进行分析，该分析可同时定量多达 25 种二十碳烷类介质。MIA 和 C 之间没有差异。与 MSA 和 C 相比，MSA 和 COPD 患者的 6-酮、PGE2、LTB4、11-12 EET 和 AA 水平较高，而 LXA4、11DHyTxB2、11HETE 和 8,9EET 水平较低（p < 0.05）。我们的研究表明，通过质谱分析 EBC 是混合的，与 MIA 和对照相比，MSA 和 COPD 的反应相似。
