一种 bZIP 蛋白 VIP1 是拟南芥中渗透压感应信号的调节剂。
A bZIP protein, VIP1, is a regulator of osmosensory signaling in Arabidopsis.
机构信息
Asian Natural Environmental Science Center, University of Tokyo, Nishitokyo-shi, Tokyo 188-0002, Japan.
出版信息
Plant Physiol. 2012 May;159(1):144-55. doi: 10.1104/pp.112.197020. Epub 2012 Mar 27.
Abstract
Abscisic acid is a stress-related phytohormone that has roles in dehydration and rehydration. In Arabidopsis (Arabidopsis thaliana), two genes that inactivate abscisic acid, CYP707A1 and CYP707A3, are rapidly up-regulated upon rehydration. The factors that regulate CYP707A1/3 are not well characterized. We expressed a bZIP protein, VIP1, as a green fluorescent protein fusion protein in Arabidopsis and found that the nuclear localization of VIP1 was enhanced within 10 min after rehydration. A yeast one-hybrid assay revealed that the amino-terminal region of VIP1 has transcriptional activation potential. In a transient reporter assay using Arabidopsis protoplasts, VIP1 enhanced the promoter activities of CYP707A1/3. In gel shift and chromatin immunoprecipitation analyses, VIP1 directly bound to DNA fragments of the CYP707A1/3 promoters. Transgenic plants expressing VIP1-green fluorescent protein were found to overexpress CYP707A1/3 mRNAs. The time course of nuclear-cytoplasmic shuttling of VIP1 was consistent with the time courses of the expression of CYP707A1/3. These results suggest that VIP1 functions as a regulator of osmosensory signaling in Arabidopsis.
摘要
脱落酸是一种与应激相关的植物激素，在脱水和再水合过程中发挥作用。在拟南芥（Arabidopsis thaliana）中，两个使脱落酸失活的基因 CYP707A1 和 CYP707A3 在再水合后迅速上调。但调节 CYP707A1/3 的因素尚未得到很好的描述。我们将 bZIP 蛋白 VIP1 表达为拟南芥中的绿色荧光蛋白融合蛋白，发现 VIP1 的核定位在再水合后 10 分钟内增强。酵母单杂交测定表明，VIP1 的氨基末端区域具有转录激活潜能。在使用拟南芥原生质体的瞬时报告基因测定中，VIP1 增强了 CYP707A1/3 的启动子活性。在凝胶迁移和染色质免疫沉淀分析中，VIP1 直接与 CYP707A1/3 启动子的 DNA 片段结合。表达 VIP1-绿色荧光蛋白的转基因植物被发现过表达 CYP707A1/3 mRNA。VIP1 的核质穿梭的时间过程与 CYP707A1/3 的表达时间过程一致。这些结果表明，VIP1 作为拟南芥中渗透感应信号的调节剂发挥作用。
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