Thompson Mitchell G, Moore William M, Hummel Niklas F C, Pearson Allison N, Barnum Collin R, Scheller Henrik V, Shih Patrick M
Joint BioEnergy Institute, Emeryville, CA, USA.
Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Biodes Res. 2020 May 26;2020:8189219. doi: 10.34133/2020/8189219. eCollection 2020.
is an important tool in plant biotechnology due to its natural ability to transfer DNA into the genomes of host plants. Genetic manipulations of have yielded considerable advances in increasing transformational efficiency in a number of plant species and cultivars. Moreover, there is overwhelming evidence that modulating the expression of various mediators of virulence can lead to more successful plant transformation; thus, the application of synthetic biology to enable targeted engineering of the bacterium may enable new opportunities for advancing plant biotechnology. In this review, we highlight engineering targets in both and plant hosts that could be exploited more effectively through precision genetic control to generate high-quality transformation events in a wider range of host plants. We then further discuss the current state of and plant engineering with regard to plant transformation and describe how future work may incorporate a rigorous synthetic biology approach to tailor strains of used in plant transformation.
由于其具有将DNA转移到宿主植物基因组中的天然能力，它是植物生物技术中的一种重要工具。对其进行基因操作已在提高许多植物物种和品种的转化效率方面取得了相当大的进展。此外，有大量证据表明，调节各种毒力介质的表达可导致更成功的植物转化；因此，应用合成生物学对该细菌进行靶向工程改造可能为推进植物生物技术带来新机遇。在本综述中，我们重点介绍了该细菌和植物宿主中的工程靶点，通过精确的基因控制可以更有效地利用这些靶点，从而在更广泛的宿主植物中产生高质量的转化事件。然后，我们进一步讨论了该细菌和植物工程在植物转化方面的现状，并描述了未来的工作如何可能采用严格的合成生物学方法来定制用于植物转化的该细菌菌株。
