应用 13C 磁共振波谱成像对脑 2-酮异己酸代谢进行成像。
Imaging cerebral 2-ketoisocaproate metabolism with hyperpolarized (13)C magnetic resonance spectroscopic imaging.
机构信息
Danish Research Centre for Magnetic Resonance, Copenhagen University Hospital, Hvidovre, Denmark.
出版信息
J Cereb Blood Flow Metab. 2012 Aug;32(8):1508-14. doi: 10.1038/jcbfm.2012.34. Epub 2012 Mar 28.
Abstract
The branched chain amino acid transaminase (BCAT) has an important role in nitrogen shuttling and glutamate metabolism in the brain. The purpose of this study was to describe the cerebral distribution and metabolism of hyperpolarized 2-keto[1-(13)C]isocaproate (KIC) in the normal rat using magnetic resonance modalities. Hyperpolarized KIC is metabolized to [1-(13)C]leucine (leucine) by BCAT. The results show that KIC and its metabolic product, leucine, are present at imageable quantities 20 seconds after end of KIC administration throughout the brain. Further, significantly higher metabolism was observed in hippocampal regions compared with the muscle tissue. In conclusion, the cerebral metabolism of hyperpolarized KIC is imaged and hyperpolarized KIC may be a promising substrate for evaluation of cerebral BCAT activity in conjunction with neurodegenerative disease.
摘要
支链氨基酸转氨酶 (BCAT) 在大脑中的氮穿梭和谷氨酸代谢中具有重要作用。本研究的目的是使用磁共振技术描述正常大鼠中极化 2-酮[1-(13)C]异己酸 (KIC) 的脑分布和代谢。极化 KIC 被 BCAT 代谢为 [1-(13)C]亮氨酸 (亮氨酸)。结果表明，KIC 及其代谢产物亮氨酸在 KIC 给药结束后 20 秒内可在整个大脑中以可成像的量存在。此外，与肌肉组织相比，在海马区观察到更高的代谢。总之，极化 KIC 的脑代谢可以成像，极化 KIC 可能是一种很有前途的底物，可用于结合神经退行性疾病评估大脑中的 BCAT 活性。
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