Thakur J S, Agarwal R K, Kharya M D
Department of Pharmaceutical Sciences, Dr. Hari Singh Gour Central University, Sagar-470 003, India.
Indian J Pharm Sci. 2011 May;73(3):271-5. doi: 10.4103/0250-474X.93510.
The present paper describes enhancement of lignans, phyllanthin and hypophyllanthin present in Phyllanthus amarus plant. Supplementation was done to enhance secondary metabolites by modifying the media and treatment with different growth promoters and abiotic elicitors to increase the content of hepatoprotective bioactives in immobilized cell cultures, after incubation for 21 days. MS medium was supplemented with gibberellic acid and to make whole process commercially viable, when coconut water, sugarcane juice and water-melon extract were treated, it was revealed that watermelon extract, enhances maximum phyllanthin and hypophyllanthin yield followed by sugarcane juice, coconut water and gibberellic acid after estimation by HPTLC. The present method was found to be accurate, economical and viable to enhance the content of phyllanthin and hypophyllanthin in P. amarus for large-scale commercial production.
本文描述了余甘子植物中木脂素、叶下珠素和去氢叶下珠素的含量提高情况。通过改良培养基以及用不同生长促进剂和非生物诱导剂处理来提高次生代谢产物，以增加固定化细胞培养物中具有肝脏保护作用的生物活性成分的含量，培养21天后进行检测。在MS培养基中添加赤霉素，为使整个过程具有商业可行性，对椰子水、甘蔗汁和西瓜提取物进行处理后，通过高效薄层色谱法（HPTLC）测定发现，西瓜提取物能使叶下珠素和去氢叶下珠素的产量提高最多，其次是甘蔗汁、椰子水和赤霉素。结果表明，本方法准确、经济且可行，可用于大规模商业生产中提高余甘子中叶下珠素和去氢叶下珠素的含量。
