The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
Biomaterials. 2010 Oct;31(30):7620-30. doi: 10.1016/j.biomaterials.2010.06.049. Epub 2010 Jul 24.
Efficacious hemostatic agents have significant potential for use in rapid exsanguinating hemorrhage control by emergency medical technician or military medic nowadays. Unfortunately, the topical hemostats currently available in market still have various disadvantages. In this study, a series of macroporous chitosan coated mesoporous silica xerogel beads (CSSX) with good biocompatibility were developed. They consisted of mesoporous silica xerogel cores and chitosan layers with macroporous structure by using modified sol-gel process and PEG molecular imprinting technique. The textural properties of the CSSX beads were optimized by in vitro and in vivo evaluation for promoting blood clotting and the results indicated that the prepared CSSX beads can significantly accelerate the contact activation pathway of coagulation cascade and produce desirable hemostasis, with the best efficiency from the CSSX prepared with 2% chitosan and 5% PEG. Furthermore, these CSSX beads were observed to create no exothermic reaction and the subsequential tissue thermal injury by histological examination, and exhibited no obvious cytotoxicity even after 7 days. The results of the present study forward CSSX bead as a safe hemostatic system and present a platform for further optimization studies of materials with enhanced hemostatic capabilities for specific injury types.
如今，有效的止血剂在急救医疗技术员或军医快速控制失血方面具有重要的应用潜力。不幸的是，目前市场上的局部止血剂仍然存在各种缺点。在这项研究中，开发了一系列具有良好生物相容性的大孔壳聚糖涂覆介孔硅干凝胶珠（CSSX）。它们由介孔硅干凝胶核和壳聚糖层组成，具有大孔结构，通过改良的溶胶-凝胶法和 PEG 分子印迹技术制备。通过体外和体内评估来优化 CSSX 珠的结构特性，以促进血液凝结，结果表明，所制备的 CSSX 珠可显著加速凝血级联的接触激活途径，并产生理想的止血效果，其中壳聚糖和 PEG 的浓度分别为 2%和 5%时效率最佳。此外，通过组织学检查观察到这些 CSSX 珠不会引起放热反应和随后的组织热损伤，即使在 7 天后也没有明显的细胞毒性。本研究结果表明 CSSX 珠是一种安全的止血系统，并为进一步优化具有增强特定损伤类型止血能力的材料提供了一个平台。
