Arsalan Mani, Woitek Felix, Adams Volker, Linke Axel, Barten Markus J, Dhein Stefan, Walther Thomas, Mohr Friedrich-Wilhelm, Garbade Jens
Department of Cardiac Surgery, Kerckhoff Klinik, Bad Nauheim, Benekestr. 2-8, 61231 Bad Nauheim,, Germany.
ISRN Cardiol. 2012;2012:483407. doi: 10.5402/2012/483407. Epub 2012 Mar 4.
Introduction. The existence of human cardiac stem cells (hCSC) and their regenerative capacity are not fully defined. The aim of this study was to identify and analyse the distribution of hCSCs by flow cytometry (FCM). Methods. Tissue samples from the left ventricle (LV) and the appendages of the right atrium (RA) and left atrium (LA) were taken during cardiac surgery. Mononuclear cells (MNCs) were isolated, labelled for the stem-cell-marker c-kit and hematopoietic-lineage markers and analysed by FCM. Results. HCSCs could be isolated from the RA, LA, and LV without significant quantitative difference between both atria (A) (RA 4.80 ± 1.76% versus LA 4.99 ± 1.69% of isolated MNCs, P = 0.922). The number of hCSCs was significantly higher in both atria compared to the left ventricle (A 4.90 ± 1.29% versus LV 0.62 ± 0.14% of isolated MNCs, P = 0.035). Conclusion. The atria contain a higher concentration of hCSC than the left ventricle. HCSCs located in the atria could serve as an endogenous source for heart regeneration.
引言。人类心脏干细胞（hCSC）的存在及其再生能力尚未完全明确。本研究的目的是通过流式细胞术（FCM）鉴定并分析hCSC的分布。方法。在心脏手术期间采集左心室（LV）以及右心房（RA）和左心房（LA）附属结构的组织样本。分离单核细胞（MNC），用干细胞标志物c-kit和造血谱系标志物进行标记，并通过FCM进行分析。结果。可从RA、LA和LV中分离出hCSC，两个心房之间无显著数量差异（分离的MNC中，RA为4.80±1.76%，LA为4.99±1.69%，P = 0.922）。与左心室相比，两个心房中的hCSC数量显著更高（分离的MNC中，心房为4.90±1.29%，LV为0.62±0.14%，P = 0.035）。结论。心房中hCSC的浓度高于左心室。位于心房的hCSC可作为心脏再生的内源性来源。
