用于分离人血浆和汗液中血管紧张素及其代谢物的高效液相色谱法。
High-performance liquid chromatography for the separation of angiotensin and its metabolites in human plasma and sweat.
作者信息
Hermann K, Ring J, Phillips M I
机构信息
Department of Dermatology, Ludwig Maximilians University Münich, West Germany.
出版信息
J Chromatogr Sci. 1990 Oct;28(10):524-8. doi: 10.1093/chromsci/28.10.524.
PMID:2246350
Abstract
A reversed-phase high-performance liquid chromatography (HPLC) method with gradient elution for the separation of angiotensin peptides is described. The highly reproducible method allows the base-line separation of angiotensin peptides with UV detection at 225 nm. This chromatographic methodology in combination with radioimmunoassay (RIA) is used for the characterization of angiotensin peptides in human plasma and sweat.
摘要
描述了一种用于分离血管紧张素肽的具有梯度洗脱的反相高效液相色谱（HPLC）方法。该高度可重现的方法能够在225 nm处进行紫外检测，实现血管紧张素肽的基线分离。这种色谱方法与放射免疫分析（RIA）相结合，用于表征人血浆和汗液中的血管紧张素肽。
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