Wang Fa-Sheng, Chen Hui, Wu Zhao-Yang, Lin Jian-Hua
Department of Orthopedics, Fujian Medical University, China.
Asian Pac J Cancer Prev. 2011;12(12):3229-34.
Osteosarcoma is the most common primary bone malignancy with a notorious feature of high metastasis. KISS1 has been identified as a putative human metastasis suppressor gene in numerous types of cancer. This study was aimed to evaluate the relationship between expression of KISS1 and invasion ability in osteosarcoma cell lines, and the relationships between KISS1 expression levels and prognosis of clinical cases.
Expression levels of KISS1 in 3 types of osteosarcoma cell lines (MG-63, Saos-2 and U-2 OS) and a normal osteoblast cell line (hF-OB 1.19) were examined using semi-quantitative RT-PCR and immunochemistry staining. Transwell assays were used to detect the cell invasion ability. The osteosarcoma cell lines and specimen sections of osteosarcoma together with control were immuno-stained with KISS1 antibody. The relationship between the clinical data and the expression of KISS1 was evaluated.
KISS1 mRNA expression was moderate in U-2 OS, weak in Saos-2 and lost in MG-63. Transwell assays displayed a gradually increased aggressive phenomenon in osteosarcoma cell lines U-2 OS, Saos-2 and MG-63. However, a contrary conclusion was obtained with clinical specimen, a higher positive rate of KISS1 expression being displayed in osteosarcoma patients, especially in metastastic cases, compared to the benign osteochondroma patients. Furthermore, significant earlier distant metastasis was observed in KISS1 positive than negative cases.
KISS1 expression levels were found to be decreased with the increasing aggressive ability in osteosarcoma cell lines. However, expression of KISS1 positively correlated with metastasis in osteosarcoma patients.
骨肉瘤是最常见的原发性骨恶性肿瘤，具有高转移这一恶名昭著的特征。KISS1已被确定为多种癌症中一种假定的人类转移抑制基因。本研究旨在评估骨肉瘤细胞系中KISS1表达与侵袭能力之间的关系，以及KISS1表达水平与临床病例预后之间的关系。
采用半定量逆转录聚合酶链反应（RT-PCR）和免疫化学染色检测3种骨肉瘤细胞系（MG-63、Saos-2和U-2 OS）及一种正常成骨细胞系（hF-OB 1.19）中KISS1的表达水平。采用Transwell实验检测细胞侵袭能力。用KISS1抗体对骨肉瘤细胞系和骨肉瘤标本切片以及对照进行免疫染色。评估临床数据与KISS1表达之间的关系。
KISS1 mRNA在U-2 OS中表达中等，在Saos-2中表达较弱，在MG-63中缺失。Transwell实验显示骨肉瘤细胞系U-2 OS、Saos-2和MG-63中侵袭现象逐渐增加。然而，临床标本得出了相反的结论，与良性骨软骨瘤患者相比，骨肉瘤患者中KISS1表达的阳性率更高，尤其是在转移病例中。此外，观察到KISS1阳性病例比阴性病例出现远处转移的时间明显更早。
发现骨肉瘤细胞系中KISS1表达水平随侵袭能力增强而降低。然而，骨肉瘤患者中KISS1的表达与转移呈正相关。
