Enzyme Research Team, RIKEN Biomass Engineering Program, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Biomacromolecules. 2012 May 14;13(5):1383-9. doi: 10.1021/bm300089a. Epub 2012 Apr 13.
We developed a facile and quick ethanol-based method for preparing silk nanoparticles and then fabricated a biodegradable and biocompatible dual-drug release system based on silk nanoparticles and the molecular networks of silk hydrogels. Model drugs incorporated in the silk nanoparticles and silk hydrogels showed fast and constant release, respectively, indicating successful dual-drug release from silk hydrogel containing silk nanoparticles. The release behaviors achieved by this dual-drug release system suggest to be regulated by physical properties (e.g., β-sheet contents and size of the silk nanoparticles and network size of the silk hydrogels), which is an important advantage for biomedical applications. The present silk-based system for dual-drug release also demonstrated no significant cytotoxicity against human mesenchymal stem cells (hMSCs), and thus, this silk-based dual-drug release system has potential as a versatile and useful new platform of polymeric materials for various types of dual delivery of bioactive molecules.
我们开发了一种简便的基于乙醇的方法来制备丝纳米粒子，然后基于丝纳米粒子和丝水凝胶的分子网络构建了一种可生物降解和生物相容的双药物释放系统。包埋在丝纳米粒子和丝水凝胶中的模型药物分别表现出快速和恒速释放，表明含有丝纳米粒子的丝水凝胶实现了双药物释放。这种双药物释放系统的释放行为可通过物理性质（例如，丝纳米粒子的β-折叠含量和粒径以及丝水凝胶的网络尺寸）进行调节，这是其在生物医学应用中的一个重要优势。本研究中的丝基双药物释放系统对人骨髓间充质干细胞（hMSCs）也没有明显的细胞毒性，因此，这种丝基双药物释放系统有望成为用于各种类型生物活性分子的双递药的通用且有用的新型聚合物材料平台。
