Liu Ye, Gill Elisabeth, Shery Huang Yan Yan
Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, UK, CB2 1PZ.
Future Sci OA. 2017 Mar 2;3(2):FSO173. doi: 10.4155/fsoa-2016-0084. eCollection 2017 Jun.
A plethora of 3D and microfluidics-based culture models have been demonstrated in the recent years with the ultimate aim to facilitate predictive models for pharmaceutical development. This article summarizes to date the progress in the microfluidics-based tissue culture models, including organ-on-a-chip and vasculature-on-a-chip. Specific focus is placed on addressing the question of what kinds of 3D culture and system complexities are deemed desirable by the biological and biomedical community. This question is addressed through analysis of a research survey to evaluate the potential use of microfluidic cell culture models among the end users. Our results showed a willingness to adopt 3D culture technology among biomedical researchers, although a significant gap still exists between the desired systems and existing 3D culture options. With these results, key challenges and future directions are highlighted.
近年来，已经展示了大量基于3D和微流控的培养模型，其最终目标是促进用于药物开发的预测模型。本文总结了迄今为止基于微流控的组织培养模型的进展，包括芯片上器官和芯片上血管系统。特别关注的是解决生物和生物医学领域认为何种3D培养和系统复杂性是理想的这一问题。通过对一项研究调查的分析来解决这个问题，以评估终端用户对微流控细胞培养模型的潜在用途。我们的结果表明生物医学研究人员愿意采用3D培养技术，尽管期望的系统与现有的3D培养选项之间仍然存在显著差距。基于这些结果，突出了关键挑战和未来方向。
