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山兰乙醇提取物及其活性部位对人黑色素瘤 HM3KO 细胞的抗增殖和促凋亡作用。

Antiproliferative and Proapoptotic Effects of Labisia pumila Ethanol Extract and Its Active Fraction in Human Melanoma HM3KO Cells.

机构信息

School of Biosciences and Biotechnology, Faculty of Science and Technology, National University of Malaysia, 43600 Bangi, Malaysia.

出版信息

Evid Based Complement Alternat Med. 2012;2012:123470. doi: 10.1155/2012/123470. Epub 2012 Mar 8.

DOI:10.1155/2012/123470
PMID:22474490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3310196/
Abstract

The present study was to determine the anticancer potential of Labisia pumila in in vitro models. Results from the study revealed that ethanol extract of L. pumila was more cytotoxic against HM3KO cells while having reduced effects on nonmalignant cells as compared to aqueous and hexane extracts. Thus, ethanol extract was selected to be further separated by using the bioassay-guided fractionation method to give an active fraction, SF2Lp. Results obtained from the flow cytometry analysis showed that SF2Lp was able to arrest the HM3KO cell cycle at the G1 phase, while morphological findings from AO-EB nuclear staining assays along with the Apoptotic Index confirmed the induction of apoptosis by SF2Lp in HM3KO cells. Results from the mechanistic study further revealed that SF2Lp treatment was able to concurrently increase the expression level of p53 and pro-apoptotic protein Bax and also reduce the expression level of anti-apoptotic protein BCl-2 in HM3KO cells, directly contributing to the increase in Bax/Bcl-2 ratio. These findings, therefore, suggested that L. pumila was able to inhibit HM3KO cell growth possibly by arresting the cell cycle at G1 phase and inducing apoptosis in HM3KO cells via the up- and down-regulation of Bax/Bcl-2 protein, mediated through a p53-dependent pathway.

摘要

本研究旨在确定蝴蝶菜（Labisia pumila）在体外模型中的抗癌潜力。研究结果表明，与水提物和正己烷提取物相比，蝴蝶菜的乙醇提取物对 HM3KO 细胞具有更强的细胞毒性，而对非恶性细胞的影响较小。因此，选择乙醇提取物进一步通过生物活性导向分离方法进行分离，得到活性部分 SF2Lp。流式细胞术分析结果表明，SF2Lp 能够将 HM3KO 细胞周期阻滞在 G1 期，而 AO-EB 核染色检测的形态学结果以及凋亡指数证实了 SF2Lp 在 HM3KO 细胞中诱导凋亡。机制研究结果进一步表明，SF2Lp 处理能够同时增加 p53 和促凋亡蛋白 Bax 的表达水平，并降低 HM3KO 细胞中抗凋亡蛋白 Bcl-2 的表达水平，直接导致 Bax/Bcl-2 比值增加。这些发现表明，蝴蝶菜可能通过将细胞周期阻滞在 G1 期并通过上调和下调 Bax/Bcl-2 蛋白诱导 HM3KO 细胞凋亡来抑制 HM3KO 细胞的生长，这是通过 p53 依赖性途径介导的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ecb/3310196/16d88ccb9072/ECAM2012-123470.010.jpg
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本文引用的文献

1
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J Biosci Bioeng. 2010 Mar;109(3):291-6. doi: 10.1016/j.jbiosc.2009.08.478. Epub 2009 Sep 18.
2
Cytotoxic triterpenoid saponins from the roots of Ardisia crenata.朱砂根根部的细胞毒性三萜皂苷
J Asian Nat Prod Res. 2008 Sep-Oct;10(9-10):833-9. doi: 10.1080/10286020802102568.
3
Targeted manipulation of apoptosis in cancer treatment.癌症治疗中对细胞凋亡的靶向操控。
文化习俗对一个多民族发展中国家乳腺癌风险、就诊时分期及预后的影响。
Oncol Lett. 2021 Nov;22(5):806. doi: 10.3892/ol.2021.13067. Epub 2021 Sep 23.
4
Shoot Multiplication and Callus Induction of var. as Influenced by Different Plant Growth Regulators Treatments and Its Polyphenolic Activities Compared with the Wild Plant.不同植物生长调节剂处理对 var. 增殖和愈伤组织诱导的影响及其与野生植物多酚活性的比较。
Molecules. 2021 May 27;26(11):3229. doi: 10.3390/molecules26113229.
5
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6
Antiproliferative and apoptosis inducing effect of essential oil extracted from (J.) Smith leaves.从（J.）史密斯叶中提取的精油的抗增殖和诱导凋亡作用。
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8
Plants as a source of anti-cancer agents.植物作为抗癌药物的来源。
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9
A simple technique for quantifying apoptosis in 96-well plates.一种用于在96孔板中定量细胞凋亡的简单技术。
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10
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