Department of Microbiology and Immunology, University of Maryland School of Medicine, HSF-1, Suite 380, 685 West Baltimore Street, Baltimore, MD 21201, USA.
Evid Based Complement Alternat Med. 2012;2012:589256. doi: 10.1155/2012/589256. Epub 2012 Mar 7.
Rheumatoid arthritis (RA) is a chronic inflammatory disease affecting the joints that can lead to deformities and disability. The prolonged use of conventionally used drugs is associated with severe adverse reactions. Therefore, safer and less expensive therapeutic products are continually being sought. Huo-Luo-Xiao-Ling dan (HLXL), a traditional Chinese herbal mixture, and its modified versions possess anti-arthritic activity. In this paper, we examined the influence of modified HLXL on two of the key mediators of arthritic inflammation and tissue damage, namely, chemokines and matrix-metalloproteinases (MMPs) in the rat adjuvant-induced arthritis (AA) model of RA. We treated arthritic Lewis rats with HLXL (2.3 g/kg) by daily gavage beginning at the onset of AA. The control rats received the vehicle. At the peak phase of AA, rats were sacrificed and their draining lymph node cells (LNC) and spleen adherent cells (SAC) were tested. The HLXL-treated rats showed a significant reduction in the levels of chemokines (RANTES, MCP-1, MIP-1α, and GRO/KC), MMPs (MMP 2 and 9), as well as cytokines (IL-6 and IL-17) that induce them, compared to the control vehicle-treated rats. Thus, HLXL controls arthritis in part by suppressing the mediators of immune pathology, and it might offer a promising alternative/adjunct treatment for RA.
类风湿性关节炎（RA）是一种影响关节的慢性炎症性疾病，可导致畸形和残疾。长期使用常规药物会引起严重的不良反应。因此，人们一直在寻求更安全、更便宜的治疗产品。 Huo-Luo-Xiao-Ling 丹（HLXL）是一种中药复方，其改良版本具有抗关节炎活性。在本文中，我们研究了改良 HLXL 对两种关键的关节炎炎症和组织损伤介质的影响，即趋化因子和基质金属蛋白酶（MMPs）在大鼠佐剂性关节炎（AA）模型中的作用。我们通过每日灌胃，从 AA 发病开始，用 HLXL（2.3g/kg）治疗关节炎性 Lewis 大鼠。对照组大鼠给予载体。在 AA 的高峰期，处死大鼠，检测其引流淋巴结细胞（LNC）和脾贴壁细胞（SAC）。与对照组大鼠相比，HLXL 治疗组大鼠的趋化因子（RANTES、MCP-1、MIP-1α 和 GRO/KC）、MMPs（MMP2 和 9）以及诱导它们的细胞因子（IL-6 和 IL-17）水平显著降低。因此，HLXL 通过抑制免疫病理学介质来控制关节炎，它可能为 RA 提供一种有前途的替代/辅助治疗方法。
