Rajaiah Rajesh, Lee David Y-W, Ma Zhongze, Fan Arthur Y, Lao Lixing, Fong Harry H S, Berman Brian M, Moudgil Kamal D
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
J Ethnopharmacol. 2009 May 4;123(1):40-4. doi: 10.1016/j.jep.2009.02.032. Epub 2009 Mar 4.
HLXL is a traditional Chinese medicine that has long been used in folk medicine for the treatment of chronic inflammatory diseases. However, the precise immunological mechanisms by which HLXL mediates its anti-inflammatory activity are not fully defined.
To determine the effects of HLXL on antigen-specific immune parameters in adjuvant-induced inflammation model in the Lewis rat.
Rats were fed daily with either HLXL (2.3g/kg) or vehicle (water) beginning 3 days before subcutaneous injection of heat-killed Mycobacterium tuberculosis H37Ra (Mtb), and then continued for another 6 days. After 9 days of Mtb injection, the draining lymph node cells were tested for T cell proliferative and cytokine responses against mycobacterial heat-shock protein 65 (Bhsp65). Moreover, sera were tested for anti-Bhsp65 antibodies and nitric oxide (NO).
HLXL-treated rats showed reduced T cell proliferative response to Bhsp65 compared to control rats. Furthermore, HLXL suppressed IL-17 response but enhanced IL-10 response without much effect on IFN-gamma. HLXL treatment also reduced the levels of anti-Bhsp65 antibodies but not that of NO.
HLXL feeding modulated both the cellular and the humoral immune response to Bhsp65 favoring an anti-inflammatory milieu for the suppression of adjuvant-induced inflammation.
黄连细辛汤是一种传统中药，长期以来一直用于民间医学治疗慢性炎症性疾病。然而，黄连细辛汤介导其抗炎活性的确切免疫机制尚未完全明确。
确定黄连细辛汤对佐剂诱导的Lewis大鼠炎症模型中抗原特异性免疫参数的影响。
在皮下注射热灭活结核分枝杆菌H37Ra（Mtb）前3天开始，大鼠每天喂食黄连细辛汤（2.3g/kg）或赋形剂（水），并持续6天。注射Mtb 9天后，检测引流淋巴结细胞对分枝杆菌热休克蛋白65（Bhsp65）的T细胞增殖和细胞因子反应。此外，检测血清中的抗Bhsp65抗体和一氧化氮（NO）。
与对照大鼠相比，黄连细辛汤处理的大鼠对Bhsp65的T细胞增殖反应降低。此外，黄连细辛汤抑制IL-17反应，但增强IL-10反应，对IFN-γ影响不大。黄连细辛汤治疗还降低了抗Bhsp65抗体水平，但对NO水平无影响。
喂食黄连细辛汤可调节对Bhsp65的细胞免疫和体液免疫反应，有利于形成抗炎环境以抑制佐剂诱导的炎症。
