Kumar E P, Mukherjee Radhika, Senthil R, Parasuraman S, Suresh B
Department of Pharmacology, JSS College of Pharmacy, Ootacamund 643001, India.
ISRN Pharmacol. 2012;2012:941068. doi: 10.5402/2012/941068. Epub 2012 Mar 5.
Objective. The objective of this work was to study the risk factors of ischaemic heart disease (IHD) in rural populations of the Nilgiris, south India, with stress on the various social habits and oxidant stress. Methods. A total of 72 patients with cardiovascular disease (CVD) and 12 healthy volunteers were screened. Forty-seven patients with CVD (intervention group) and 10 healthy volunteers (control group) were randomly selected for the study. Written informed consent was obtained from all the participants, and their demographic details were collected. A 6 mL blood sample was collected from each of the participants, and the serum was separated in the samples. The levels of enzymic (superoxide dismutase, catalase) and nonenzymic antioxidants (ascorbic acid) in the plasma were determined biochemically. The level of thiobarbituric acid species (TBARS), which is a predictor of lipid peroxidation, was measured. Results. The participants of the study were stratified as according to demographic and social variables. The values of all the antioxidants and TBARS were statistically compared. Significantly reduced antioxidant levels and increased TBARS levels were found in the intervention group compared with the control group. The results suggest that the lowered antioxidant level may be a result of the oxidant stress of the disease. Statistically significant differences were not found in the antioxidant and TBARS levels when comparing smokers versus nonsmokers, alcoholics versus nonalcoholics, and vegetarians versus nonvegetarians. Conclusion. The major causes of CVD amongst the rural populations of the Nilgiris, south India, are preventable causes such as smoking and high fat intake, all of which cause oxidative stress, as seen in our study through various serum markers.
目的。本研究旨在探讨印度南部尼尔吉里斯农村地区缺血性心脏病（IHD）的危险因素，重点关注各种社会习惯和氧化应激。方法。共筛选了72例心血管疾病（CVD）患者和12名健康志愿者。随机选取47例CVD患者（干预组）和10名健康志愿者（对照组）进行研究。所有参与者均签署了书面知情同意书，并收集了他们的人口统计学详细信息。从每位参与者采集6毫升血液样本，并分离样本中的血清。采用生化方法测定血浆中酶性抗氧化剂（超氧化物歧化酶、过氧化氢酶）和非酶性抗氧化剂（抗坏血酸）的水平。测量了作为脂质过氧化指标的硫代巴比妥酸物质（TBARS）水平。结果。根据人口统计学和社会变量对研究参与者进行分层。对所有抗氧化剂和TBARS的值进行统计学比较。与对照组相比，干预组的抗氧化剂水平显著降低，TBARS水平升高。结果表明，抗氧化剂水平降低可能是疾病氧化应激的结果。在比较吸烟者与非吸烟者、酗酒者与非酗酒者、素食者与非素食者时，抗氧化剂和TBARS水平未发现统计学显著差异。结论。印度南部尼尔吉里斯农村地区CVD的主要原因是可预防的因素，如吸烟和高脂肪摄入，所有这些都会导致氧化应激，正如我们在研究中通过各种血清标志物所观察到的那样。
