Bastani Abdolhossein, Rajabi Sadegh, Daliran Afshin, Saadat Habibollah, Karimi-Busheri Feridoun
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran 1985717443, Iran.
Cardiovascular Research Center, Modares Hospital, Shahid Beheshti University of Medical Sciences, Tehran 1998734383, Iran.
Biomed Rep. 2018 Oct;9(4):327-332. doi: 10.3892/br.2018.1130. Epub 2018 Jul 17.
Formation of atherosclerotic plaques is the major cause of coronary artery disease (CAD). Several lines of study have revealed the role of oxidative stress in CAD pathogenesis. In the present study the aim was to investigate the oxidative and antioxidative markers in CAD patients and a control population. The study sample comprised of acute coronary syndrome (ACS) patients, chronic CAD patients and healthy controls (n=30/group). Blood samples of patients and control subjects were collected to measure the concentrations of reduced glutathione (GSH), malondialdehyde (MDA) and the percentage of MDA release as well as the activity of erythrocyte glutathione peroxidase (GPx) and total antioxidant capacity (TAC) of plasma. All parameters were measured by spectrophotometric methods. Additionally, oxidant/antioxidant status was compared between CAD patients with single, double or triple-vessel stenosis and in comparison with controls. The results indicated a significant increase in MDA level and the percentage of MDA release (P<0.05), and a marked decrease in GSH concentration (P<0.0001), TAC (P<0.0001) and the activity of erythrocyte GPx (P<0.0001) in the patient groups compared controls. ACS patients exhibited a similar pattern of data when compared with the chronic CAD group. Similar results were also observed when chronic CAD patients with single, double or triple vessel stenosis and controls were compared. The present study indicates that the acute form of CAD is more susceptible to oxidative damage, suggesting that use of antioxidant therapy may be warranted to ameliorate oxidative stress in this condition.
动脉粥样硬化斑块的形成是冠状动脉疾病（CAD）的主要原因。多项研究揭示了氧化应激在CAD发病机制中的作用。在本研究中，目的是调查CAD患者和对照组人群中的氧化和抗氧化标志物。研究样本包括急性冠状动脉综合征（ACS）患者、慢性CAD患者和健康对照组（每组n = 30）。采集患者和对照受试者的血样，以测量还原型谷胱甘肽（GSH）、丙二醛（MDA）的浓度以及MDA释放百分比，以及红细胞谷胱甘肽过氧化物酶（GPx）的活性和血浆总抗氧化能力（TAC）。所有参数均通过分光光度法测量。此外，比较了单支、双支或三支血管狭窄的CAD患者与对照组之间的氧化/抗氧化状态。结果表明，与对照组相比，患者组中MDA水平和MDA释放百分比显著升高（P < 0.05），而GSH浓度（P < 0.0001）、TAC（P < 0.0001）和红细胞GPx活性（P < 0.0001）显著降低。与慢性CAD组相比，ACS患者呈现出类似的数据模式。当比较单支、双支或三支血管狭窄的慢性CAD患者与对照组时，也观察到了类似结果。本研究表明，CAD的急性形式更容易受到氧化损伤，这表明在这种情况下可能有必要使用抗氧化疗法来减轻氧化应激。
