Srivastava Kumar Chandan, Austin Ravi David, Shrivastava Deepti, Sethupathy S, Rajesh S
Division of Oral Medicine and Radiology, Rajah Muthiah Dental College and Hospital, Annamalai University, Annamalai Nagar, Chidambaram, India.
Contemp Clin Dent. 2012 Jul;3(3):271-6. doi: 10.4103/0976-237X.103617.
Imbalances between the oxidant -antioxidant status have been implicated in the pathogenesis of several diseases, including cancer.
The aim of this study was to evaluate the extent of lipid peroxidation and antioxidants in the venous blood samples of oral squamous cell carcinoma patients of different Clinicopathologic stages in comparison with the healthy controls.
A Case control study was designed in a hospital (Rajah Muthiah Dental College and Hospital, Annamalai University) based setting.
Twenty new histopathologically proven oral carcinoma patients, and equal number of age, sex and habit matched healthy subjects were recruited for this study. Their blood samples were subjected to evaluation of Thiobarbituric Acid Reactive Substances (TBARS) and antioxidant enzymes, namely, superoxide dismutase (SOD), Catalase (CAT) reduced glutathione (GSH) and glutathione peroxidase (GPx) using spectrophotometric methods.
The data are expressed as mean±SD. The statistical comparisons were performed by independent Student's t-test and One Way ANOVA. P value <0.05 was considered statistically significant. Karl Pearson correlation was performed for the biochemical parameters within the group and between the groups. For statistically significant correlations, linear regression was performed.
Significant enhanced lipid peroxidation (P<0.001) with decrease in antioxidants (P<0.001) was observed in the venous blood of oral squamous cell carcinoma patients as compared with the healthy controls. Accordingly, significant (P<0.001) pattern of progression in TBARS levels was observed at various clinical stages of patients. (GSH) showed significant (P<0.01) negative correlation with TBARS and positive correlation (P<0.001) with SOD. On linear regression analysis, GSH showed significance for SOD (P<0.001), GPx, CAT and TBARS (P<0.01). It was also found that, 70% of variance in SOD can be attributed to the influence of GSH alone.
Enhanced lipid peroxidation and compromised antioxidant defense in plasma indicate development of oxidative stress. Amongst the antioxidant enzymes, (GSH) appears to have a profound role in carcinogenesis.
氧化-抗氧化状态失衡与包括癌症在内的多种疾病的发病机制有关。
本研究旨在评估不同临床病理分期的口腔鳞状细胞癌患者静脉血样本中脂质过氧化程度和抗氧化剂水平，并与健康对照进行比较。
在一家医院（安纳马莱大学拉贾·穆西亚牙科学院和医院）进行了一项病例对照研究。
招募了20名经组织病理学证实的新口腔癌患者，以及数量相等、年龄、性别和习惯匹配的健康受试者。采用分光光度法对他们的血样进行硫代巴比妥酸反应性物质（TBARS）和抗氧化酶的评估，即超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、还原型谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx）。
数据以平均值±标准差表示。采用独立样本t检验和单因素方差分析进行统计比较。P值<0.05被认为具有统计学意义。对组内和组间的生化参数进行卡尔·皮尔逊相关性分析。对于具有统计学意义的相关性，进行线性回归分析。
与健康对照相比，口腔鳞状细胞癌患者静脉血中脂质过氧化显著增强（P<0.001），抗氧化剂水平降低（P<0.001）。相应地，在患者的各个临床阶段观察到TBARS水平有显著的（P<0.001）进展模式。（GSH）与TBARS呈显著（P<0.01）负相关，与SOD呈正相关（P<0.001）。在线性回归分析中，GSH对SOD（P<0.001）、GPx、CAT和TBARS（P<0.01）具有显著意义。还发现，SOD中70%的变异仅可归因于GSH的影响。
血浆中脂质过氧化增强和抗氧化防御受损表明氧化应激的发展。在抗氧化酶中，（GSH）似乎在致癌过程中具有重要作用。
