School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bangalore, India.
Pharm Biol. 2012 Aug;50(8):948-53. doi: 10.3109/13880209.2011.649858. Epub 2012 Apr 6.
Phyllanthus (Euphorbiaceae) species are traditionally well-known for their medicinal properties including hepatoprotective activity.
The study assessed the hepatoprotective and antioxidant activities of 11 Phyllanthus species, P. amarus Schumach., P. urinaria L., P. debilis Klein ex Willd, P. tenellus Roxb., P. virgatus G. Forst., P. maderaspatensis L., P. reticulatus Poir., P. polyphyllus Willd., P. emblica L., P. indofischerii Bennet. and P. acidus (L.) Skeels.
The dried leaves and stems of each plant species were extracted in methanol and successively in water. The extracts were screened for hepatoprotective activity at a concentration of 50 µg/mL against tert-butyl hydroperoxide (t-BH) induced toxicity in HepG2 cells. Seven extracts from five species that showed hepatoprotective activity were assessed for their 50% effective concentration (EC₅₀) values and their antioxidant activity using a DPPH assay. Phyllanthin and hypophyllanthin contents were also determined in these Phyllanthus species.
The methanol extracts of P. polyphyllus, P. emblica and P. indofischeri showed high levels of hepatoprotective activity with EC₅₀ values of 12, 19 and 28 µg/mL and IC₅₀ of 3.77, 3.38 and 5.8 µg/mL for DPPH scavenging activity respectively against an IC₅₀ of 3.69 µg/mL for ascorbic acid. None of these activities could be attributed to phyllanthin and hypophyllanthin.
The hepatoprotective and antioxidant activities of P. indofischeri are demonstrated for the first time in literature. The study also confirms the hepatoprotective and antioxidant activities of leaves of P. emblica and P. polyphyllus. The molecule(s) responsible for the activities is being investigated.
叶下珠科（Euphorbiaceae）物种以其药用特性而闻名，包括肝保护活性。
本研究评估了 11 种叶下珠属植物，即显脉叶下珠（Phyllanthus amarus Schumach.）、利尿叶下珠（P. urinaria L.）、柔弱叶下珠（P. debilis Klein ex Willd.）、纤细叶下珠（P. tenellus Roxb.）、纤绿叶下珠（P. virgatus G. Forst.）、余甘子（P. maderaspatensis L.）、网脉叶下珠（P. reticulatus Poir.）、多花山芝麻（P. polyphyllus Willd.）、余甘子（P. emblica L.）、印度叶下珠（P. indofischerii Bennet.）和酸叶下珠（P. acidus（L.）Skeels.）的肝保护和抗氧化活性。
将每种植物的干燥叶和茎分别用甲醇和水提取。将提取物在 50 µg/mL 浓度下筛选对 tert-butyl hydroperoxide（t-BH）诱导的 HepG2 细胞毒性的肝保护活性。对显示肝保护活性的五种植物的七种提取物进行 50%有效浓度（EC₅₀）值评估，并使用 DPPH 测定法评估其抗氧化活性。还测定了这些叶下珠属植物中的叶下珠素和隐叶下珠素含量。
多花山芝麻、余甘子和印度叶下珠的甲醇提取物表现出高水平的肝保护活性，EC₅₀ 值分别为 12、19 和 28 µg/mL，DPPH 清除活性的 IC₅₀ 值分别为 3.77、3.38 和 5.8 µg/mL，而 DPPH 清除活性的 IC₅₀ 值为 3.69 µg/mL。这些活性都不能归因于叶下珠素和隐叶下珠素。
印度叶下珠的肝保护和抗氧化活性首次在文献中得到证实。该研究还证实了余甘子和多花山芝麻叶的肝保护和抗氧化活性。正在研究负责这些活性的分子。
