Fragasso Alberto, Mannarella Clara, Ciancio Angela, Scarciolla Oronzo, Nuzzolese Nicoletta, Clemente Rocco, Vitullo Eustachio, Sacco Andrea
Ospedale Madonna delle Grazie, ASM, Matera, Italy.
ScientificWorldJournal. 2012;2012:128182. doi: 10.1100/2012/128182. Epub 2012 Mar 12.
Measurement of serum cobalamin (Cbl) levels is the standard investigation for assessing vitamin B12 deficiency. Falsely increased values of Cbl can be caused by alcoholic liver disease. Measurement of total vitamin B12 serum levels might be misleading in alcoholics, because a tissue metabolic deficiency is possible even with normal serum Cbl levels. Holotranscobalamin (HoloTC), the Cbl metabolically active fraction, is considered as a better index of vitamin B12 deficiency.
For assessing vitamin B12 status, we evaluated 22 adult alcoholic male patients by measuring in parallel serum Cbl, serum folate and red blood cell folate levels, HoloTC levels by the AxSYM assay. RESULTS. HoloTC values were reduced in 3 alcoholics with borderline-low Cbl values. Significant positive correlations were found between serum Cbl and HoloTC levels, serum Cbl and gamma-glutamyl transpeptidase (GGT).
HoloTC measurement is a useful option for assessing vitamin B12 status in alcoholics, particularly in the subjects with borderline Cbl values and may be considered an early marker of vitamin B12 deficiency.
血清钴胺素（Cbl）水平的测定是评估维生素B12缺乏症的标准检查方法。酒精性肝病可导致Cbl值假性升高。在酗酒者中，测定血清总维生素B12水平可能会产生误导，因为即使血清Cbl水平正常，也可能存在组织代谢缺乏的情况。全转钴胺素（HoloTC）是Cbl的代谢活性部分，被认为是评估维生素B12缺乏症的更好指标。
为评估维生素B12状态，我们通过使用AxSYM检测法同时检测血清Cbl、血清叶酸和红细胞叶酸水平以及HoloTC水平，对22名成年男性酗酒患者进行了评估。结果。3名Cbl值处于临界低水平的酗酒者的HoloTC值降低。血清Cbl与HoloTC水平、血清Cbl与γ-谷氨酰转肽酶（GGT）之间存在显著正相关。
HoloTC检测是评估酗酒者维生素B12状态的一种有用方法，特别是对于Cbl值处于临界水平的受试者，并且可被视为维生素B12缺乏的早期标志物。
