Mayers Jonathan R, Audhya Anjon
Department of Biomolecular Chemistry; University of Wisconsin-Madison Medical School; Madison, WI USA.
Commun Integr Biol. 2012 Jan 1;5(1):50-6. doi: 10.4161/cib.18208.
Vesicle-mediated cargo transport within the endomembrane system requires precise coordination between adaptor molecules, which recognize sorting signals on substrates, and factors that promote changes in membrane architecture. At endosomal compartments, a set of protein complexes collectively known as the ESCRT machinery sequesters transmembrane cargoes that harbor a ubiquitin modification and packages them into vesicles that bud into the endosome lumen. Several models have been postulated to describe this process. However, consensus in the field remains elusive. Here, we discuss recent findings regarding the structure and function of the ESCRT machinery, highlighting specific roles for ESCRT-0 and ESCRT-III in regulating cargo selection and vesicle formation.
内膜系统中囊泡介导的货物运输需要衔接分子（识别底物上的分选信号）与促进膜结构变化的因子之间精确协调。在内体区室，一组统称为ESCRT机制的蛋白质复合物隔离带有泛素修饰的跨膜货物，并将它们包装到向内体腔中出芽的囊泡中。已经提出了几种模型来描述这一过程。然而，该领域仍未达成共识。在这里，我们讨论了关于ESCRT机制的结构和功能的最新发现，强调了ESCRT-0和ESCRT-III在调节货物选择和囊泡形成中的特定作用。
