Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
Talanta. 2012 May 15;93:320-5. doi: 10.1016/j.talanta.2012.02.041. Epub 2012 Feb 22.
Poly(l-arginine)/graphene composite film modified electrode was successfully prepared via a facile one-step electrochemical method and used for simultaneous determination of uric acid (UA), xanthine (XA) and hypoxanthine (HX). The electrochemical behaviors of UA, XA and HX at the modified electrode were studied by cyclic voltammetry and differential pulse voltammetry (DPV), and showed that the modified electrode exhibited excellent electrocatalytic activity toward the oxidation of the three compounds. The calibration curves for UA, XA and HX were obtained over the range of 0.10-10.0, 0.10-10.0 and 0.20-20.0 μM by DPV, respectively and the detection limits for UA, XA and HX were 0.05, 0.05 and 0.10 μM (S/N=3), respectively. With good selectivity and high sensitivity, the modified electrode has been applied to simultaneous determination of UA, XA and HX in human urine with satisfactory result.
聚（L-精氨酸）/石墨烯复合膜修饰电极通过简便的一步电化学方法成功制备，并用于同时测定尿酸（UA）、黄嘌呤（XA）和次黄嘌呤（HX）。通过循环伏安法和差分脉冲伏安法（DPV）研究了修饰电极上 UA、XA 和 HX 的电化学行为，结果表明修饰电极对三种化合物的氧化具有优异的电催化活性。通过 DPV 分别在 0.10-10.0、0.10-10.0 和 0.20-20.0 μM 范围内获得了 UA、XA 和 HX 的校准曲线，UA、XA 和 HX 的检测限分别为 0.05、0.05 和 0.10 μM（S/N=3）。修饰电极具有良好的选择性和高灵敏度，已成功应用于人尿液中 UA、XA 和 HX 的同时测定，结果令人满意。
