聚甘氨酸/还原氧化石墨烯修饰玻碳电极同时测定黄嘌呤和次黄嘌呤。

Simultaneous Determination of Xanthine and Hypoxanthine Using Polyglycine/rGO-Modified Glassy Carbon Electrode.

机构信息

School of Health & Social Care, Shanghai Urban Construction Vocational College, Shanghai 201401, China.

College of Sciences, Shanghai University, Shanghai 200444, China.

出版信息

Molecules. 2023 Feb 2;28(3):1458. doi: 10.3390/molecules28031458.

A novel electrochemical sensor was developed for selective and sensitive determination of xanthine (XT) and hypoxanthine (HX) based on polyglycine (p-Gly) and reduced graphene oxide (rGO) modified glassy carbon electrode (GCE). A mixed dispersion of 7 μL of 5 mM glycine and 1 mg/mL GO was dropped on GCE for the fabrication of p-Gly/rGO/GCE, followed by cyclic voltammetric sweeping in 0.1 M phosphate buffer solution within -0.45~1.85 V at a scanning rate of 100 mV·s. The morphological and electrochemical features of p-Gly/rGO/GCE were investigated by scanning electron microscopy and cyclic voltammetry. Under optimal conditions, the linear relationship was acquired for the simultaneous determination of XT and HX in 1-100 μM. The preparation of the electrode was simple and efficient. Additionally, the sensor combined the excellent conductivity of rGO and the polymerization of Gly, demonstrating satisfying simultaneous sensing performance to both XT and HX.

基于聚甘氨酸（p-Gly）和还原氧化石墨烯（rGO）修饰的玻碳电极（GCE），开发了一种用于选择性和灵敏测定黄嘌呤（XT）和次黄嘌呤（HX）的新型电化学传感器。将 7 μL 的 5 mM 甘氨酸和 1 mg/mL GO 的混合分散体滴在 GCE 上，用于制备 p-Gly/rGO/GCE，然后在 0.1 M 磷酸盐缓冲溶液中以 100 mV·s 的扫描速率在-0.45~1.85 V 之间进行循环伏安扫描。通过扫描电子显微镜和循环伏安法研究了 p-Gly/rGO/GCE 的形貌和电化学特性。在最佳条件下，可在 1-100 μM 范围内同时测定 XT 和 HX 的线性关系。电极的制备简单高效。此外，该传感器结合了 rGO 的优异导电性和 Gly 的聚合，对 XT 和 HX 均表现出令人满意的同时传感性能。