Abakumov M A, Goldt A E, Sokolsky-Papkov M, Zorkina Y A, Baklaushev V P, Goodilin E A, Kabanov A V, Chekhonin V P
Department of Medical Nanobiotechnology, Medicobiological Faculty, Russian State Medical University, Ministry of Health Care and Social Development of Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2011 Oct;151(6):726-30. doi: 10.1007/s10517-011-1426-5.
We propose a method for obtaining superparamagnetic nanoparticles based on iron oxide and their water suspensions. The structure and size of nanoparticles were confirmed by transmission electron microscopy, dynamic light scattering, and X-ray diffraction analysis. The nanoparticles also contained a fluorescent dye Dil C18. Cytotoxicity of obtained aqueous suspension was studied by MTT assay; low toxicity of nanoparticles was demonstrated. High T2-relaxivity of nanoparticles allows using them as a contrast agent for MRI. After incubation of cerebellar sections with nanoparticles vectorized with antibodies to antigen AMVB1, specific visualization of blood vessels was detected.
我们提出了一种基于氧化铁及其水悬浮液制备超顺磁性纳米颗粒的方法。通过透射电子显微镜、动态光散射和X射线衍射分析确定了纳米颗粒的结构和尺寸。纳米颗粒还含有荧光染料Dil C18。通过MTT法研究了所得水悬浮液的细胞毒性；结果表明纳米颗粒具有低毒性。纳米颗粒的高T2弛豫率使其能够用作磁共振成像（MRI）的造影剂。在用抗抗原AMVB1抗体进行载体化处理的纳米颗粒孵育小脑切片后，检测到了血管的特异性可视化。
