INRB-LNIV, Laboratório Nacional de Investigação Veterinária , Estrada de Benfica 701, 1549-011 Lisboa, Portugal.
J Agric Food Chem. 2012 May 2;60(17):4227-34. doi: 10.1021/jf205186y. Epub 2012 Apr 20.
The use of nitrofurans as veterinary drugs has been banned from intensive animal production in the European Union (EU) since 1993. The objective of the present study was to evaluate the accumulation and depletion of furaltadone and nifursol and their side-chain metabolites 5-methylmorpholino-3-amino-2-oxazolidinone (AMOZ) and 3,5-dinitrosalicylic acid hydrazide (DNSAH) in eggs after administration of therapeutic and subtherapeutic doses of the drugs to laying hens during three consecutive weeks. LC-MS/MS, with positive and negative electrospray ionization methods, was used for the determination of parent compounds and metabolites in yolk and egg white and was validated according to criteria established by Commission Decision 2002/657/EC. The decision limit (CCα) and the detection capability (CCβ) of the analytical methodology for metabolites were 0.1 and 0.5 μg/kg for AMOZ and 0.3 and 0.9 μg/kg for DNSAH, respectively. For the parent compounds, CCα and CCβ were 0.9 and 2.0 μg/kg for furaltadone and 1.3 and 3.1 μg/kg for nifursol, respectively. The data obtained show that the parent compounds are much less persistent than their side-chain metabolites in either yolk or egg white. Between the studied metabolites, AMOZ is the most persistent and could be detected in either yolk or egg white three weeks following withdrawal from treatment.
自 1993 年以来，欧盟（EU）已禁止在集约化动物生产中使用硝基呋喃类药物作为兽药。本研究的目的是评估在连续三周内给产蛋母鸡治疗和亚治疗剂量的药物后，呋喃他酮和硝呋索尔及其侧链代谢物 5-甲基吗啉-3-氨基-2-恶唑烷酮（AMOZ）和 3,5-二硝基水杨酸腙（DNSAH）在鸡蛋中的积累和消除情况。采用 LC-MS/MS 结合正、负离子电喷雾电离法，对蛋黄和蛋清中的母体化合物和代谢物进行了测定，并按照欧盟委员会第 2002/657/EC 号决定规定的标准进行了验证。代谢物分析方法的检测限（CCα）和检测能力（CCβ）分别为 AMOZ 的 0.1 和 0.5μg/kg，DNSAH 的 0.3 和 0.9μg/kg。对于母体化合物，呋喃他酮的 CCα和 CCβ分别为 0.9 和 2.0μg/kg，硝呋索尔的 CCα和 CCβ分别为 1.3 和 3.1μg/kg。所得数据表明，在蛋黄或蛋清中，母体化合物的持久性均远低于其侧链代谢物。在所研究的代谢物中，AMOZ 的持久性最强，停药后三周仍可在蛋黄或蛋清中检测到。
