患有特发性复发性流产的墨西哥夫妇中男性的Y染色体微缺失

Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss.

作者信息

Piña-Aguilar Raul E, Martínez-Garza Sandra G, Kohls Graciela, Vargas-Maciel Marco A, Vázquez de Lara Luis G, González-Ortega Claudia, Cancino-Villarreal Patricia, Gutiérrez-Gutiérrez Antonio M

机构信息

Institute of Sciences in Human Reproduction, León, Mexico.

出版信息

J Obstet Gynaecol Res. 2012 Jun;38(6):912-7. doi: 10.1111/j.1447-0756.2011.01809.x. Epub 2012 Apr 9.

DOI:10.1111/j.1447-0756.2011.01809.x

PMID:22486969

Abstract

AIM

To analyze the presence of Y chromosome microdeletions in males of Mexican couples with idiopathic recurrent pregnancy losses (RPL).

METHODS

Seventy-one males from couples with RPL and 66 fertile males as controls were studied. DNA was isolated from peripheral lymphocytes and used to run multiplex polymerase chain reactions. Regions AZFa (sY84, sY86), AZFb (sY127, sY134) and AZFc (sY254, sY255) of the Y chromosome were analyzed according to valid guidelines recommended by the European Academy of Andrology and the European Molecular Genetics Quality Network. Also, the sequence tagged sites (STSs): DYS262 (sY67), DYS220 (sY129), DYF85S1 (sY150), DYF86S1 (sY152) and DYF87S1 (sY153) were included in order to analyze STSs previously reported as deleted. A power analysis to support our simple size was performed.

RESULTS

Results show an absence of Y chromosome microdeletions in males of couples with RPL and controls with an acceptable statistical power.

CONCLUSION

The study did not show an association of recurrent pregnancy loss and Y chromosome microdeletions in Mexican male partners. Based on the results, the study of Y chromosome microdeletions in couples with RPL is not considered clinically relevant.

摘要

目的

分析患有特发性复发性流产（RPL）的墨西哥夫妇中男性的Y染色体微缺失情况。

方法

研究了71名来自复发性流产夫妇的男性以及66名可育男性作为对照。从外周血淋巴细胞中分离DNA，并用于进行多重聚合酶链反应。根据欧洲男科学会和欧洲分子遗传学质量网络推荐的有效指南，分析Y染色体的AZFa（sY84、sY86）、AZFb（sY127、sY134）和AZFc（sY254、sY255）区域。此外，还纳入了序列标签位点（STSs）：DYS262（sY67）、DYS220（sY129）、DYF85S1（sY150）、DYF86S1（sY152）和DYF87S1（sY153），以分析先前报道为缺失的STSs。进行了功效分析以支持我们的样本量。