Rodrigues M M, McCarey B E, Waring G O, Hidayat A A, Kruth H S
Department of Ophthalmology, University of Maryland, Baltimore.
Refract Corneal Surg. 1990 Jan-Feb;6(1):32-7.
Synthetic materials are being evaluated for their potential long-term use in corneal refractive surgery. Clinical and histopathologic studies were performed with polymethylmethacrylate (PMMA) and polysulfone intracorneal lenses in rhesus monkey eyes that were followed for up to 3 years. The 5 mm diameter lenses were placed in the deep posterior corneal stroma of four eyes. Fine, polychromatic crystalline deposits formed a layer posterior to the implants. Enucleated eyes had the corneas either frozen for histochemistry or fixed for electron microscopy. Special stains included oil red 0 and filipin on fresh frozen tissue. The cornea, with a PMMA intracorneal lens showed myriad crystalline aggregates in the deep corneal stroma behind the implant. These crystalline deposits stained positively with oil red 0 and with filipin indicating the presence of neutral fat as well as unesterified cholesterol. The polysulfone implant showed no evidence of crystalline deposits histologically but was surrounded by homogeneous aggregates that could represent nonspecific reaction to the lens material or protein deposits. Electron microscopy of all four corneas revealed dissolved lipid aggregates and laminated electron dense material that were most abundant posterior to the implant where the keratocytes appeared disintegrated. The PMMA lens appeared to induce lipid keratopathy.
正在评估合成材料在角膜屈光手术中长期使用的潜力。对恒河猴眼睛植入聚甲基丙烯酸甲酯（PMMA）和聚砜角膜内透镜进行了临床和组织病理学研究，随访长达3年。将直径5毫米的透镜植入四只眼睛的角膜基质深层后部。在植入物后方形成了一层细小的多色结晶沉积物。摘除的眼球的角膜要么冷冻用于组织化学检查，要么固定用于电子显微镜检查。特殊染色包括新鲜冷冻组织上的油红O和菲律宾菌素。植入PMMA角膜内透镜的角膜在植入物后方的角膜基质深层显示出无数结晶聚集体。这些结晶沉积物用油红O和菲律宾菌素染色呈阳性，表明存在中性脂肪以及未酯化胆固醇。聚砜植入物在组织学上没有结晶沉积物的证据，但被均匀的聚集体包围，这些聚集体可能代表对透镜材料的非特异性反应或蛋白质沉积物。对所有四只角膜的电子显微镜检查显示，溶解的脂质聚集体和层状电子致密物质在植入物后方最为丰富，此处角膜细胞似乎已解体。PMMA透镜似乎诱发了脂质角膜病变。
