单体扩散进入镰状血红蛋白的聚合物结构域。
Monomer diffusion into polymer domains in sickle hemoglobin.
作者信息
Cho M R, Ferrone F A
机构信息
Department of Physics and Atmospheric Science, Drexel University, Philadelphia, Pennsylvania 19104.
出版信息
Biophys J. 1990 Oct;58(4):1067-73. doi: 10.1016/S0006-3495(90)82449-8.
The gelation of sickle hemoglobin includes the formation of spherulitic arrays of polymers, known as polymer domains, which are an intrinsic result of the polymer formation mechanism. We have observed the diffusion of monomers into domains as they form, which substantially increases the total concentration of hemoglobin within the domain. The maximum total concentration attained is comparable with the pellet concentration of 0.5-0.55 g/cm3 obtained in sedimentation experiments. The half time for this process is approximately 50 s for domains of 25 microns radius, and is approximately independent of temperature. The shape of the diffusion progress curves as well as the deduced diffusion constants, and their weak temperature dependence are consistent with a simple model of hemoglobin monomer diffusion into the domain.
镰状血红蛋白的凝胶化包括形成聚合物的球晶阵列，即所谓的聚合物结构域，这是聚合物形成机制的内在结果。我们观察到单体在结构域形成时扩散进入结构域，这大大增加了结构域内血红蛋白的总浓度。达到的最大总浓度与沉降实验中获得的0.5 - 0.55 g/cm³的沉淀浓度相当。对于半径为25微米的结构域，这个过程的半衰期约为50秒，并且大致与温度无关。扩散过程曲线的形状以及推导的扩散常数，及其对温度的微弱依赖性与血红蛋白单体扩散进入结构域的简单模型一致。
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