血红蛋白 S 溶液和凝胶的准弹性激光光散射
Quasi-elastic laser light scattering from solutions and gels of hemoglobin S.
作者信息
Kam Z, Hofrichter J
出版信息
Biophys J. 1986 Nov;50(5):1015-20. doi: 10.1016/S0006-3495(86)83544-5.
Abstract
Quasi-elastic light scattering has been used to examine solutions and gels of deoxyhemoglobin S. The autocorrelation function is found to decay with a characteristic exponential relaxation which can be ascribed to the diffusion of monomer (64,000 molecular weight) hemoglobin S molecules. In the absence of polymers, the relaxation time is in good agreement with previous measurements of the diffusion coefficient for solutions of normal human hemoglobin. In the presence of the polymer phase, a large (greater than 200-fold) increase in the scattered intensity is observed but no contribution to the decay of the autocorrelation function from the motion of the aligned polymer phase can be detected. Heterodyning between the time-independent scattering amplitude from the polymers and the time-dependent scattering of the diffusing monomers results in a twofold increase in the relaxation time arising from monomer diffusion.
摘要
准弹性光散射已被用于检测脱氧血红蛋白S的溶液和凝胶。发现自相关函数以特征指数弛豫衰减，这可归因于单体（分子量64,000）血红蛋白S分子的扩散。在没有聚合物的情况下，弛豫时间与先前对正常人血红蛋白溶液扩散系数的测量结果吻合良好。在聚合物相存在的情况下，观察到散射强度大幅增加（大于200倍），但未检测到对齐聚合物相的运动对自相关函数衰减有贡献。聚合物的与时间无关的散射振幅和扩散单体的与时间有关的散射之间的外差作用导致单体扩散引起的弛豫时间增加两倍。
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